Department of Urology, The First Affiliated Hospital of Soochow University, Jiangsu, China.
Department of Urology, Jining No, 1 People's Hospital, Jining, China.
Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):695-702. doi: 10.1080/21691401.2020.1725024.
Renal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154 in RCC tissues and cell lines were detected using qRT-PCR assay. To examine cellular viability of ACHN and CAKI-1 cells, cell counting kit-8 (CCK-8) assay was exploited here. Flow cytometric analysis was conducted to examine apoptosis. Cell mobility was valued through wound healing assays. Western blotting was applied for examination of proteins related to proliferation, apoptosis, migration and Wnt/β-catenin/Notch. LINC01939 was down-regulated in RCC tissues. LINC01939 overexpression impeded proliferation and migration, and induced apoptosis. Further study found that the overexpression of LINC01939 strongly suppressed miR-154 expression. Then, the inhibiting effect of overexpressed LINC01939 on proliferation and mobility and the promoting role of LINC01939 in apoptosis were abolished by the combination of miR-154 mimic. Finally, we found that overexpressed LINC01939 inactivated Wnt/β-catenin and Notch through suppressing miR-154. Up-regulation of LINC01939 inhibited proliferation and migration of RCC cells by down-regulating miR-154.
肾细胞癌（RCC）被广泛认为是一种泌尿系统恶性肿瘤。长链非编码 RNA 1939（LINC01939）是一种新型的 lncRNA，在 RCC 中发现下调。因此，我们着手探讨 LINC01939 在 RCC 中的作用和调节机制。采用 qRT-PCR 检测 RCC 组织和细胞系中的 LINC01939 和 miR-154。利用细胞计数试剂盒-8（CCK-8）检测 ACHN 和 CAKI-1 细胞的细胞活力。通过流式细胞术分析检测细胞凋亡。通过划痕愈合实验评估细胞迁移能力。应用 Western blot 检测与增殖、凋亡、迁移和 Wnt/β-catenin/Notch 相关的蛋白。LINC01939 在 RCC 组织中下调。LINC01939 过表达抑制增殖和迁移，并诱导凋亡。进一步研究发现，LINC01939 的过表达强烈抑制 miR-154 的表达。然后，通过 miR-154 模拟物的组合，消除了过表达 LINC01939 对增殖和迁移的抑制作用以及 LINC01939 对凋亡的促进作用。最后，我们发现过表达 LINC01939 通过抑制 miR-154 使 Wnt/β-catenin 和 Notch 失活。LINC01939 的上调通过下调 miR-154 抑制 RCC 细胞的增殖和迁移。
