Department of Cardiovascular Medicine, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan.
Department of Cellular and Molecular Medicine, Chiba University Graduate School of Medicine, Chiba, Japan.
BMC Cardiovasc Disord. 2020 Mar 5;20(1):116. doi: 10.1186/s12872-020-01396-2.
Left ventricular reverse remodeling (LVRR) has been detected in non-ischemic dilated cardiomyopathy (NIDCM) patients following optimal treatment. However, its prediction with only conventional modalities is often difficult. This study sought to examine whether RNA sequencing (RNA-seq) of myocardium tissue samples could predict LVRR in NIDCM.
A total of 17 advanced NIDCM patients with left ventricular ejection fraction (LVEF) below 30% who underwent cardiac biopsy from Left ventricle (LV) were prospectively recruited. They received optimal treatment and followed with echocardiogram every 6 months. Based on LVRR status after 12 months of treatment, patients were divided into the reverse remodeling (RR) or non-RR group. Tissue samples were analyzed by RNA-seq, and a functional analysis of differentially expressed genes was carried out.
There were eight and nine patients in the RR and non-RR groups, respectively. No difference was found in age, sex, disease duration, LV end-diastolic diameter, and LVEF between the two groups. There were 155 genes that were differentially expressed between the two groups. Nicotinamide adenine dinucleotide ubiquinone oxidoreductase subunit (NDUF)S5 and Growth arrest and DNA-damage-inducible protein (GADD)45G, along with several genes related to the mitochondrial respiratory chain and ribosome, were significantly downregulated in the RR as compared to the non-RR group.
GADD45G and NDUFS5 are potential biomarkers for LVRR in patients with advanced NIDCM.
在接受最佳治疗后，非缺血性扩张型心肌病（NIDCM）患者的左心室逆向重构（LVRR）已被检测到。然而，仅通过常规方式预测其效果往往较为困难。本研究旨在探讨心肌组织样本的 RNA 测序（RNA-seq）是否可预测 NIDCM 患者的 LVRR。
共前瞻性招募了 17 名接受左心室（LV）心内膜活检的 LVEF 低于 30%的晚期 NIDCM 患者。他们接受了最佳治疗，并在每 6 个月进行一次超声心动图检查。根据治疗 12 个月后的 LVRR 状态，患者被分为逆向重构（RR）或非逆向重构（non-RR）组。对组织样本进行 RNA-seq 分析，并对差异表达基因进行功能分析。
RR 组和 non-RR 组分别有 8 名和 9 名患者。两组间在年龄、性别、病程、LV 舒张末期直径和 LVEF 方面无差异。两组间有 155 个基因表达存在差异。RR 组中烟酰胺腺嘌呤二核苷酸泛醌氧化还原酶亚基（NDUF）S5 和生长停滞和 DNA 损伤诱导蛋白（GADD）45G 以及几个与线粒体呼吸链和核糖体相关的基因表达明显下调。
GADD45G 和 NDUFS5 可能是晚期 NIDCM 患者 LVRR 的潜在生物标志物。
