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海带多糖可增强免疫抑制小鼠体内自然杀伤细胞的活性。

Laminarin enhances the activity of natural killer cells in immunosuppressed mice.

作者信息

Zhu Xiaobao, Zhu Renzhong, Jian Zhiyuan, Yu Hong

机构信息

Department of Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin, China.

Second Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China.

出版信息

Cent Eur J Immunol. 2019;44(4):357-363. doi: 10.5114/ceji.2019.92784. Epub 2020 Jan 20.

DOI:10.5114/ceji.2019.92784
PMID:32140046
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7050051/
Abstract

The aim of the study was to investigate the effects of laminarin on natural killer (NK) cell cytotoxicity of immunosuppressive mice and its mechanism. Cyclophosphamide (cy) was used to make an immunosuppressive model of mice. The mice of two groups were given interventions by gavage with laminarin 500 mg/kg and 1000 mg/kg every day for 10 days. MACS was adopted to isolate spleen NK cells, and cytotoxicity of NK cells and IL-12, IFN-γ level in serum were detected in vivo. Cytotoxicity of NK92-MI cells, activating receptors (NKp30, NKp44, NKp46 and NKG2D) and perforin and granzyme B expression were detected in vitro. Compared to the normal control group, the cytotoxicity of NK cells, IL-12 and IFN-γ level in serum in the cy model group were all reduced significantly (p < 0.01). Compared to the cy model group, laminarin increased the cytotoxicity of NK cells, IL-12 and IFN-γ levels in serum significantly (p < 0.05). In vitro, laminarin increased the cytotoxicity, NKp30 and NKG2D, perforin and granzyme B expressions of NK92-MI cells (p < 0.01). This research showed that laminarin can promote NK cell cytotoxicity in immunosuppressive mice by increasing the levels of IL-12 and IFN-γ in serum and expressions of NKp30 and NKG2D, perforin and granzyme B.

摘要

本研究旨在探讨海带多糖对免疫抑制小鼠自然杀伤（NK）细胞细胞毒性的影响及其作用机制。采用环磷酰胺（Cy）建立小鼠免疫抑制模型。两组小鼠每天经口灌胃给予500mg/kg和1000mg/kg海带多糖，连续10天。采用磁珠分选法分离脾脏NK细胞，检测体内NK细胞细胞毒性及血清中IL-12、IFN-γ水平。体外检测NK92-MI细胞的细胞毒性、活化受体（NKp30、NKp44、NKp46和NKG2D）以及穿孔素和颗粒酶B的表达。与正常对照组相比，Cy模型组NK细胞细胞毒性、血清中IL-12和IFN-γ水平均显著降低（p<0.01）。与Cy模型组相比，海带多糖显著提高了NK细胞细胞毒性、血清中IL-12和IFN-γ水平（p<0.05）。体外实验中，海带多糖提高了NK92-MI细胞的细胞毒性、NKp30和NKG2D、穿孔素和颗粒酶B的表达（p<0.01）。本研究表明，海带多糖可通过提高血清中IL-12和IFN-γ水平以及NKp30和NKG2D、穿孔素和颗粒酶B的表达，促进免疫抑制小鼠NK细胞的细胞毒性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/1bad4d89c0a7/CEJI-44-39790-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/524d1fee3814/CEJI-44-39790-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/633e7d7a1bc7/CEJI-44-39790-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/dc82780b245a/CEJI-44-39790-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/2907d7a88ab9/CEJI-44-39790-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/1bad4d89c0a7/CEJI-44-39790-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/524d1fee3814/CEJI-44-39790-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/633e7d7a1bc7/CEJI-44-39790-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/dc82780b245a/CEJI-44-39790-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/2907d7a88ab9/CEJI-44-39790-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a91/7050051/1bad4d89c0a7/CEJI-44-39790-g005.jpg

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1
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Semin Immunol. 2017 Jun;31:20-29. doi: 10.1016/j.smim.2017.08.002. Epub 2017 Sep 6.
2
Role of ion channels in natural killer cell function towards cancer.离子通道在自然杀伤细胞对癌症的作用中的角色。
Discov Med. 2017 Jun;23(129):353-360.
3
Laminarin promotes anti-cancer immunity by the maturation of dendritic cells.海带多糖通过树突状细胞的成熟促进抗癌免疫。
昆布多糖的生物学特性及促进健康功能：临床前与临床研究的全面综述。
Mar Drugs. 2022 Dec 10;20(12):772. doi: 10.3390/md20120772.
4
Brown Seaweed Food Supplementation: Effects on Allergy and Inflammation and Its Consequences.褐藻食品补充剂：对过敏和炎症的影响及其后果。
Nutrients. 2021 Jul 29;13(8):2613. doi: 10.3390/nu13082613.
Oncotarget. 2017 Jun 13;8(24):38554-38567. doi: 10.18632/oncotarget.16170.
4
Targeting natural killer cells in cancer immunotherapy.在癌症免疫疗法中靶向自然杀伤细胞。
Nat Immunol. 2016 Aug 19;17(9):1025-36. doi: 10.1038/ni.3518.
5
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Stem Cell Res Ther. 2016 Jul 7;7(1):88. doi: 10.1186/s13287-016-0353-9.
6
ERK1/2, MEK1/2 and p38 downstream signalling molecules impaired in CD56 dim CD16+ and CD56 bright CD16 dim/- natural killer cells in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis patients.在慢性疲劳综合征/肌痛性脑脊髓炎患者的CD56dimCD16+和CD56brightCD16dim/-自然杀伤细胞中受损的ERK1/2、MEK1/2和p38下游信号分子。
J Transl Med. 2016 Apr 21;14:97. doi: 10.1186/s12967-016-0859-z.
7
Effect of compound Kushen injection on T-cell subgroups and natural killer cells in patients with locally advanced non-small-cell lung cancer treated with concomitant radiochemotherapy.复方苦参注射液对同步放化疗的局部晚期非小细胞肺癌患者T细胞亚群及自然杀伤细胞的影响
J Tradit Chin Med. 2016 Feb;36(1):14-8. doi: 10.1016/s0254-6272(16)30002-4.
8
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9
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J Virol. 2015 Jun;89(12):6435-41. doi: 10.1128/JVI.00435-15. Epub 2015 Apr 8.
10
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Fish Shellfish Immunol. 2014 Dec;41(2):402-6. doi: 10.1016/j.fsi.2014.09.027. Epub 2014 Sep 28.