Song Kyeongeun, Xu Li, Zhang Wei, Cai Yun, Jang Bian, Oh Junghwan, Jin Jun-O
Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai, 201508, China.
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, 48513, Korea.
Oncotarget. 2017 Jun 13;8(24):38554-38567. doi: 10.18632/oncotarget.16170.
This research evaluates the effects of laminarin on the maturation of dendritic cells and on the in vivo activation of anti-cancer immunity. In vivo treatment of C56BL/6 mice with laminarin increased the expression levels of co-stimulatory molecules and the production of pro-inflammatory cytokines in spleen dendritic cells. Laminarin enhanced ovalbumin antigen presentation in spleen dendritic cells and promoted the proliferation of OT-I and OT-II T cells. Laminarin also induced the maturation of dendritic cells in tumor-draining lymph nodes and protected interferon-γ and tumor necrosis factor-α and proliferation of OT-I and OT-II T cells in tumors. The combination treatment of laminarin and ovalbumin inhibited B16-ovallbumin melanoma tumor growth and its liver metastasis by antigen-specific immune activation, including cytotoxic T lymphocyte activation and interferon-γ production. Thus, these data demonstrated the potential of laminarin as a new and useful immune stimulatory molecule for use in cancer immunotherapy.
本研究评估了海带多糖对树突状细胞成熟及体内抗癌免疫激活的影响。用海带多糖对C56BL/6小鼠进行体内治疗，可提高脾脏树突状细胞中共刺激分子的表达水平及促炎细胞因子的产生。海带多糖增强了脾脏树突状细胞中卵清蛋白抗原的呈递，并促进了OT-I和OT-II T细胞的增殖。海带多糖还可诱导肿瘤引流淋巴结中树突状细胞的成熟，并保护肿瘤中的干扰素-γ和肿瘤坏死因子-α以及OT-I和OT-II T细胞的增殖。海带多糖与卵清蛋白联合治疗通过抗原特异性免疫激活，包括细胞毒性T淋巴细胞激活和干扰素-γ产生，抑制了B16-卵清蛋白黑色素瘤肿瘤生长及其肝转移。因此，这些数据证明了海带多糖作为一种新型且有用的免疫刺激分子用于癌症免疫治疗的潜力。
