Jiang Chen-Kai, Ma Jian-Qiang, Liu Yu-Fei, Chen Jie-Dan, Ni De-Jiang, Chen Liang
1Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, 9 South Meiling Road, Hangzhou, Zhejiang 310008 China.
2College of Horticulture and Forestry Science, Huazhong Agricultural University, 1 Shizishan Street, Hongshan District, Wuhan, Hubei 430070 China.
Hortic Res. 2020 Mar 1;7:24. doi: 10.1038/s41438-020-0247-y. eCollection 2020.
Catechins are the predominant products in tea plants and have essential functions for both plants and humans. Several genes encoding the enzymes regulating catechin biosynthesis have been identified, and the identification of single nucleotide polymorphisms (SNPs) resulting in nonsynonymous mutations within these genes can be used to establish a functional link to catechin content. Therefore, the transcriptomes of two parents and four filial offspring were sequenced using next-generation sequencing technology and aligned to the reference genome to enable SNP mining. Subsequently, 176 tea plant accessions were genotyped based on candidate SNPs using kompetitive allele-specific polymerase chain reaction (KASP). The catechin contents of these samples were characterized by high-performance liquid chromatography (HPLC), and analysis of variance (ANOVA) was subsequently performed to determine the relationship between genotypes and catechin content. As a result of these efforts, a SNP within the () gene was shown to be functionally associated with catechin content. Furthermore, the geographical and interspecific distribution of this SNP was investigated. Collectively, these results will contribute to the early evaluation of tea plants and serve as a rapid tool for accelerating targeted efforts in tea breeding.
儿茶素是茶树中的主要产物，对植物和人类都具有重要功能。已经鉴定出几个编码调节儿茶素生物合成的酶的基因，并且鉴定这些基因内导致非同义突变的单核苷酸多态性（SNP）可用于建立与儿茶素含量的功能联系。因此，使用下一代测序技术对两个亲本和四个子代的转录组进行测序，并与参考基因组比对以进行SNP挖掘。随后，基于候选SNP使用竞争性等位基因特异性聚合酶链反应（KASP）对176份茶树种质进行基因分型。这些样品的儿茶素含量通过高效液相色谱（HPLC）进行表征，随后进行方差分析（ANOVA）以确定基因型与儿茶素含量之间的关系。通过这些努力，结果表明（）基因内的一个SNP与儿茶素含量存在功能关联。此外，还研究了该SNP的地理和种间分布。总的来说，这些结果将有助于茶树的早期评估，并作为加速茶树育种目标努力的快速工具。
