一项关于人诱导多能干细胞衍生心肌细胞（iPS-CM）代谢特征分析的方案。
A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM).
作者信息
House Alisha, Fatica Erica, Shah Rohan, Stergar Jared, Pearce Ryan, Sandlers Yana
机构信息
Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States.
出版信息
MethodsX. 2019 May 29;7:100572. doi: 10.1016/j.mex.2019.05.028. eCollection 2020.
Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.
人类诱导多能干细胞衍生心肌细胞（iPSCM）领域的最新进展为心脏代谢建模、心脏病候选药物筛选和心脏毒性评估提供了一个新平台。这些工作流程需要对iPSCM进行全面功能表征。在此，我们报告了一份iPSCM代谢表征的分步方案。所描述的分析涵盖了对参与重要代谢途径的小分子代谢物的分析。
Zotero 插件