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一项关于人诱导多能干细胞衍生心肌细胞（iPS-CM）代谢特征分析的方案。

A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM).

作者信息

House Alisha, Fatica Erica, Shah Rohan, Stergar Jared, Pearce Ryan, Sandlers Yana

机构信息

Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States.

出版信息

MethodsX. 2019 May 29;7:100572. doi: 10.1016/j.mex.2019.05.028. eCollection 2020.

DOI:10.1016/j.mex.2019.05.028
PMID:32140435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7046813/
Abstract

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

摘要

人类诱导多能干细胞衍生心肌细胞（iPSCM）领域的最新进展为心脏代谢建模、心脏病候选药物筛选和心脏毒性评估提供了一个新平台。这些工作流程需要对iPSCM进行全面功能表征。在此，我们报告了一份iPSCM代谢表征的分步方案。所描述的分析涵盖了对参与重要代谢途径的小分子代谢物的分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/d82df74b0d01/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/5f77eb8d9836/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/1ead0e061dd2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/6b9249fd94ec/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/ccd8b9f8b29c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/7f8708601c59/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/b90f41db082c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/d37d95f5f901/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/9b76e1660861/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/d82df74b0d01/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/5f77eb8d9836/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/1ead0e061dd2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/6b9249fd94ec/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/ccd8b9f8b29c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/7f8708601c59/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/b90f41db082c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/d37d95f5f901/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/9b76e1660861/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c0/7046813/d82df74b0d01/gr8.jpg

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