Ervina Martha, Poerwono Hadi, Widyowati Retno, Matsunami Katsuyoshi
Doctoral Program of Pharmaceutical Sciences, Department of Pharmacognosy and Phytochemistry Faculty of Pharmacy, Universitas Airlangga, Indonesia.
Department of Pharmaceutical Biology, Faculty of Pharmacy, Widya Mandala Catholic University, Indonesia.
Biotechnol Rep (Amst). 2020 Feb 17;25:e00437. doi: 10.1016/j.btre.2020.e00437. eCollection 2020 Mar.
L. is used widely in traditional medicine for local or systemic ailments. Although studies exist on phytochemicals and potencies of Chinese and Indian cultivars of , the present study investigated antioxidant properties of wild type and its cytotoxicity against T47D cell. The ethanolic extract of the leaves was fractionated with n-hexane, ethyl acetate and water, and the secondary metabolites were obtained. The antioxidant properties were determined with IC DPPH (2,2-diphenyl-1-pycrylhydrazyl) radical and FRAP (ferric reducing antioxidant power), while the cytotoxicity was determined with the MTT method. The total phenolic (TPC) and β-sitosterol (SC) contents were also measured. The results showed that the ethyl acetate fraction had higher antioxidant and cytotoxic activities (IC 211.89 ± 10.86 and 147.90 ± 8.49 μg/mL, respectively) than others. Significant (p < 0.05) correlations were observed between TPC, ICDPPH, FRAP and ICT47D. LC-EI MS analysis of the ethyl acetate fraction revealed the steroid and triterpenoid saponins, limonoids and quercetin glycosides, which influenced the medicinal properties of the leaves. L. wild type leaf extracts are a promising natural resource for managing breast cancer.
L. 在传统医学中被广泛用于治疗局部或全身性疾病。尽管已有关于中国和印度栽培品种的植物化学成分和药效的研究，但本研究调查了L. 野生型的抗氧化特性及其对T47D细胞的细胞毒性。L. 叶片的乙醇提取物用正己烷、乙酸乙酯和水进行分离，得到次生代谢产物。通过IC DPPH（2,2-二苯基-1-苦基肼）自由基和FRAP（铁还原抗氧化能力）测定抗氧化特性，同时用MTT法测定细胞毒性。还测定了总酚（TPC）和β-谷甾醇（SC）含量。结果表明，乙酸乙酯部分的抗氧化和细胞毒性活性（IC分别为211.89±10.86和147.90±8.49μg/mL）高于其他部分。在TPC、ICDPPH、FRAP和ICT47D之间观察到显著（p<0.05）相关性。乙酸乙酯部分的LC-EI MS分析揭示了甾体和三萜皂苷、柠檬苦素和槲皮素糖苷，这些成分影响了L. 叶片的药用特性。L. 野生型叶片提取物是治疗乳腺癌的一种有前景的天然资源。
