Department of Analytical Chemistry, University of Valencia, c/Dr. Moliner, 50, 46100, Valencia, Burjassot, Spain.
Department of Inorganic Chemistry, Institute of Molecular Science (ICMOL), University of Valencia, c/Catedrático José Beltrán 2, 46980, Valencia, Paterna, Spain.
Mikrochim Acta. 2020 Mar 5;187(4):201. doi: 10.1007/s00604-020-4185-z.
A bio-metal-organic framework (bio-MOF) derived from the amino acid L-serine has been prepared in bulk form and evaluated as sorbent for the molecular recognition and extraction of B-vitamins. The functional pores of bio-MOF exhibit high amounts of hydroxyl groups jointly directing other supramolecular host-guest interactions thus providing the recognition of B-vitamins in fruit juices and energy drinks. Single-crystal X-ray diffraction studies reveal the specific B-vitamin binding sites and the existence of multiple hydrogen bonds between these target molecules and the framework. It offered unique snapshots to accomplish an efficient capture of these solutes in complex aqueous matrices. Four B-vitamins (thiamin, nicotinic acid, nicotinamide, and pyridoxine) were investigated. They were eluted from the sorbent with phosphate buffer at pH 7 and analyzed by HPLC with UV detection. The sorbent was compared with commercial C18 cartridges. Following the procedure, acceptable reproducibility (RSD values < 14%) was achieved, and the detection limits were in the range 0.4 to 1.4 ng mL. The method was applied to the analysis of energy drink and juice samples and the recoveries were between 75 and 123% in spiked beverage samples. Graphical abstractA bio-MOF as SPE sorbent was prepared and applied to the extraction of B-vitamins in fruit juices and energy drinks.
一种由 L-丝氨酸衍生的生物金属有机骨架（bio-MOF）已批量制备，并被评估为用于识别和提取 B 族维生素的吸附剂。bio-MOF 的功能孔具有大量的羟基，共同指导其他超分子主客体相互作用，从而提供了对果汁和能量饮料中 B 族维生素的识别。单晶 X 射线衍射研究揭示了特定的 B 族维生素结合位点以及这些目标分子与骨架之间存在多个氢键。它提供了独特的快照，以实现对这些复杂水溶液中溶质的有效捕获。研究了四种 B 族维生素（硫胺素、烟酸、烟酰胺和吡哆醇）。它们在 pH 7 的磷酸盐缓冲液中从吸附剂上洗脱下来，并通过 HPLC 与 UV 检测进行分析。该吸附剂与商业 C18 小柱进行了比较。按照该程序，可实现可接受的重现性（RSD 值 < 14%），检测限范围为 0.4 至 1.4 ng mL。该方法已应用于能量饮料和果汁样品的分析，在加标饮料样品中的回收率在 75%至 123%之间。
