Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou 510006, China.
Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen 518057, China.
ACS Sens. 2020 Mar 27;5(3):870-878. doi: 10.1021/acssensors.0c00219. Epub 2020 Mar 16.
The development of cost-effective and versatile sensing system for simultaneous and rapid quantitation of multiple targets is highly demanded for environmental surveillance, food safety inspection, home healthcare, etc. This work reports on (1) paper-based microarrays relying on fluorescence turn-off of carbon nanodots (CDs) for analyte recognition and (2) a stand-alone smartphone-based portable reader (SBR) installed with a custom-designed APP (SBR-App), which can accurately and reproducibly acquire fluorescence change from the microarray chip, automatically report the results, generate and share the reports via wireless network. Simultaneous detection of Hg, Pb, and Cu in the Pearl River water samples was achieved with the reported sensing system. End-user operation is limited to pipet samples to the microarray chip, insert the chip to the SBR, and open the SBR-App to acquire an image 5 min after sample introduction. There is no requirement for complicated sample pre-treatment and expensive equipment except for a smartphone. This versatile and cost-effective smartphone-based sensing system featured with reliability and simplicity is ideally suited for user- and eco-friendly point-of-need detection in resource-constrained environments.
开发经济高效且多功能的传感系统，用于同时快速定量多种目标，这对于环境监测、食品安全检测、家庭医疗保健等领域非常重要。本工作报道了（1）基于纸的微阵列，该微阵列依赖于碳纳米点（CDs）的荧光猝灭来进行分析物识别，以及（2）一种带有定制应用程序（SBR-App）的独立智能手机便携式读取器（SBR），该读取器可以准确且可重复地从微阵列芯片获取荧光变化，自动报告结果，并通过无线网络生成和共享报告。该传感系统可用于同时检测珠江水样中的 Hg、Pb 和 Cu。用户操作仅限于将样品用移液器加到微阵列芯片上，将芯片插入 SBR，然后打开 SBR-App，在样品引入 5 分钟后获取图像。除了智能手机外，该系统不需要进行复杂的样品预处理和昂贵的设备。这种多功能且经济高效的基于智能手机的传感系统具有可靠性和简单性，非常适合在资源有限的环境中进行用户友好和环保的现场检测。
