Wang D, Chen P, Jiang X C, Halpern M
Department of Biochemistry, SUNY Health Science Center, Brooklyn 11203.
Arch Biochem Biophys. 1988 Dec;267(2):459-66. doi: 10.1016/0003-9861(88)90051-3.
We have isolated a potent proteinaceous chemoattractant from aqueous washes of earthworm (Lumbricus terrestris) for garter snakes (Thamnophis sirtalis) by means of a covalent chromatography. It contained free sulfhydryl groups and showed an apparent mass of 20 kDa. The chemoattractive activity of this protein could be destroyed by heating as well as by proteolysis. Its activity could also be reversibly blocked by mixed disulfide formation with dithiodipyridine, suggesting that the free sulfhydryl(s) was essential for its function as a chemoattractant. This bioactive material had a tendency to form intermolecular crosslinked aggregates during isolation, if reducing agents were not included. Some of the high-molecular-weight aggregates cochromatographed with earthworm cuticle collagen on Ultragel AcA 34 or 44 columns. In contrast to an earlier report by D. M. Kirschenbaum, N. Schulman, and M. Halpern [1986) Proc. Natl. Acad. Sci. USA 83, 1213-1216) the purified earthworm collagen showed no chemoattractive activity to garter snakes.
我们通过共价色谱法从蚯蚓（陆正蚓）的水洗液中分离出了一种对束带蛇（美洲束带蛇）有效的蛋白质类化学引诱剂。它含有游离巯基，表观质量为20 kDa。该蛋白质的化学引诱活性可被加热以及蛋白水解破坏。其活性也可通过与二硫代二吡啶形成混合二硫键而被可逆阻断，这表明游离巯基对其作为化学引诱剂的功能至关重要。如果不加入还原剂，这种生物活性物质在分离过程中倾向于形成分子间交联聚集体。一些高分子量聚集体在Ultragel AcA 34或44柱上与蚯蚓表皮胶原蛋白共色谱。与D. M. 基尔申鲍姆、N. 舒尔曼和M. 哈尔彭[1986年，《美国国家科学院院刊》83, 1213 - 1216]早期的报告相反，纯化的蚯蚓胶原蛋白对束带蛇没有化学引诱活性。
