Kirschenbaum D M, Schulman N, Yao P, Halpern M
Comp Biochem Physiol B. 1985;82(3):447-53. doi: 10.1016/0305-0491(85)90007-0.
Heating a 0.1 N HCl solution of lyophilized garter snake, Thamnophis sirtalis, chemoattractant obtained from earthworm, Lumbricus terrestris, surface washings, in a boiling water bath, and separation by gel chromatography, gave two fractions, F2, of high molecular weight and containing all the chemoattractant activity, and F4, of low molecular weight. The activity is lost between 15 and 30 min of heating. A similarly treated 0.1 N NaOH solution of lyophilized washings gave no F2 fraction and an F4 fraction in increasing amount. All the chemoattractant activity is lost between 0 and 15 min. Separations were monitored by assays for protein and carbohydrate.
将从蚯蚓体表冲洗液中获得的冻干束带蛇（Thamnophis sirtalis）趋化因子的0.1N盐酸溶液在沸水浴中加热，然后通过凝胶色谱分离，得到两个组分，高分子量的F2组分，它含有所有趋化因子活性，以及低分子量的F4组分。加热15至30分钟之间活性丧失。对冻干冲洗液的0.1N氢氧化钠溶液进行类似处理，未得到F2组分，且F4组分的量不断增加。所有趋化因子活性在0至15分钟之间丧失。通过蛋白质和碳水化合物测定来监测分离情况。
