Payton M A, McCullough W, Roberts C F, Guest J R
J Bacteriol. 1977 Mar;129(3):1222-6. doi: 10.1128/jb.129.3.1222-1226.1977.
The activity of the overall pyruvate dehydrogenase complex was found to be similar in extracts of Aspergillus nidulans after growth on either sucrose or acetate. Eight mutants lacking the activity of this complex were found among some 200 glycolytic mutants selected for their inability to grow on sucrose. The absence of pyruvate dehydrogenase complex activity was also confirmed for a mutant, g6 (pdhA1), isolated previously. Studies with the mutants supported the existence of two unlinked genes, pdhA and pdhB, controlling the function of the complex. In vivo and in vitro complementation between mutations at the two loci were shown by the ability of forced heterokaryons to grow on sucrose and by the restoration of overall pyruvate dehydrogenase complex activity in mixed cell-free extracts. The mutations were recessive to their wild-type alleles, and the pdhA and pdhB loci were assigned to linkage groups I and V, respectively.
在以蔗糖或乙酸盐为碳源生长的构巢曲霉提取物中，发现丙酮酸脱氢酶复合体的总活性相似。在约200个因无法在蔗糖上生长而被挑选出的糖酵解突变体中，发现了8个缺乏该复合体活性的突变体。之前分离出的一个突变体g6（pdhA1）也被证实缺乏丙酮酸脱氢酶复合体活性。对这些突变体的研究支持存在两个不连锁的基因pdhA和pdhB，它们控制着该复合体的功能。通过强制异核体在蔗糖上生长的能力以及混合无细胞提取物中丙酮酸脱氢酶复合体总活性的恢复，证明了两个位点突变之间的体内和体外互补。这些突变对其野生型等位基因是隐性的，并且pdhA和pdhB位点分别被定位到连锁群I和V。
