Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.
FEBS J. 2020 Apr;287(7):1403-1428. doi: 10.1111/febs.15277. Epub 2020 Mar 25.
Pyridoxal 5'-phosphate (PLP) is an organic cofactor employed by ~ 4% of enzymes. The structure of the PLP cofactor allows for the stabilization of carbanions through resonance. A small number of PLP-dependent enzymes employ molecular oxygen as a cosubstrate. Here, we review the biological roles and possible mechanisms of these enzymes, and we observe that these enzymes are found in multiple protein families, suggesting that reaction with oxygen might have emerged de novo in several protein families and thus could be directed to emerge again through laboratory evolution experiments.
吡哆醛 5'-磷酸（PLP）是一种有机辅因子，大约有 4%的酶需要它。PLP 辅因子的结构允许通过共振稳定碳负离子。少数依赖 PLP 的酶将分子氧作为共底物。在这里，我们回顾了这些酶的生物学作用和可能的机制，并观察到这些酶存在于多个蛋白质家族中，这表明与氧的反应可能是在几个蛋白质家族中从头出现的，因此可以通过实验室进化实验再次出现。
