Int J Oral Maxillofac Implants. 2020 Mar/Apr;35(2):275-280. doi: 10.11607/jomi.7913.
Platelet concentrates are used for regenerative periodontal and implant therapy. Up to now, no study has reported the influence of smoking on platelet-rich fibrin membranes. Hence, this cross-sectional in vitro study aimed to analyze the influence of cigarette smoking on platelet morphology and fiber characteristics of both leukocyte- and platelet-rich fibrin and advanced platelet-rich fibrin membranes.
Sixty blood samples from both smokers (n = 34) and nonsmokers (n = 26) based on power analysis were collected and subjected for complete blood count and platelet morphology indices (mean platelet volume, platelet distribution width, platelet-large cell ratio, and plateletcrit). Leukocyte- and platelet-rich fibrin membrane (2,700 revolutions per minute for 12 minutes) and advanced platelet-rich fibrin membrane (1,500 revolutions per minute for 14 minutes) were prepared using a standard protocol. Thirty-two platelet-rich fibrin membranes from 16 individuals were selected randomly from the two groups and were subjected to morphologic examination using a scanning electron microscope.
Both of the groups were matched for age. Red cell counts and white cell counts showed no statistical difference between the groups. Platelet indices of smokers did show slightly higher values than the nonsmoking group. Scanning electron microscopic analysis showed variations in the fiber width and pattern among smokers in both the leukocyte- and platelet-rich fibrin and advanced platelet-rich fibrin membranes. Platelet cell morphology of the smoking group demonstrated spiky architecture, suggesting an active state, while in the nonsmoking group, the platelet cells were seen in clusters, suggesting a resting state.
Scanning electron microscopic results show that long-term cigarette smoking does affect the thickness and arrangement of fiber architecture in both leukocyte- and platelet-rich fibrin and advanced platelet-rich fibrin membranes and also could have an impact on activation of platelets.
血小板浓缩物用于再生性牙周和种植体治疗。迄今为止，尚无研究报道吸烟对富含血小板纤维蛋白膜的影响。因此，本横断面体外研究旨在分析吸烟对白细胞和血小板丰富的纤维蛋白和高级血小板丰富的纤维蛋白膜中血小板形态和纤维特征的影响。
根据功率分析，从吸烟者（n=34）和非吸烟者（n=26）中采集了 60 个血液样本，并进行了全血细胞计数和血小板形态指数（平均血小板体积、血小板分布宽度、血小板大细胞比和血小板比容）检测。使用标准方案制备白细胞和血小板丰富的纤维蛋白膜（每分钟 2700 转 12 分钟）和高级血小板丰富的纤维蛋白膜（每分钟 1500 转 14 分钟）。从两组中随机选择 32 个来自 16 个人的富含血小板的纤维蛋白膜，并使用扫描电子显微镜对其进行形态学检查。
两组的年龄相匹配。两组的红细胞计数和白细胞计数均无统计学差异。吸烟者的血小板指数略高于非吸烟者。扫描电子显微镜分析显示，吸烟者的纤维宽度和模式在白细胞和血小板丰富的纤维蛋白和高级血小板丰富的纤维蛋白膜中存在差异。吸烟组的血小板细胞形态呈刺状结构，表明处于活跃状态，而非吸烟组的血小板细胞呈簇状，表明处于静止状态。
扫描电子显微镜结果表明，长期吸烟确实会影响白细胞和血小板丰富的纤维蛋白和高级血小板丰富的纤维蛋白膜中纤维结构的厚度和排列方式，并且可能会影响血小板的激活。
