Department of Oral Health Sciences, Periodontology, KU Leuven and Dentistry, University Hospitals Leuven, Kapucijnenvoer 7, blok a - bus 07001, 3000, Leuven, Belgium.
Department of Periodontology and Oral Implantology, Faculty of Dentistry, School of Dentistry, University of Los Andes, Santiago, Chile.
Sci Rep. 2021 Mar 16;11(1):6038. doi: 10.1038/s41598-021-85736-y.
Recently, new centrifugation protocols for the preparation of platelet-rich fibrin (PRF) have been introduced in an attempt to further improve the beneficial impact of these 2nd generation platelet concentrate membranes. This in-vitro study aimed to compare the biological and physical characteristics of three types of PRF membranes using two different centrifuges with adapted relative centrifugal forces (RCF): leucocyte- and platelet-rich fibrin, advanced platelet-rich fibrin, and advanced platelet-rich fibrin. Release of growth factors, macroscopic dimensions, cellular content and mechanical properties of the respective membranes, prepared from blood of the same individual were explored. Furthermore, the impact of timing (blood draw-centrifugation and centrifugation-membrane preparation) was assessed morphologically as well as by electron microscopy scanning. No statistically significant differences amongst the three PRF modifications could be observed, neither in their release of growth factors or the cellular content, nor in clot/membrane dimensions. The difference between both centrifuges were negligible when the same g-force was used. A lower g-force, however, reduced membrane tensile strength. Timing in the preparation process had a significant impact. Adaptation of RCF only had a minimal impact on the final characteristics of PRF membranes.
最近，为了进一步提高第二代血小板浓缩物膜的有益影响，已经引入了新的用于制备富血小板纤维蛋白（PRF）的离心方案。本体外研究旨在使用两种不同的离心机（适应的相对离心力（RCF））比较三种 PRF 膜的生物学和物理特性：白细胞和血小板富纤维蛋白、先进血小板富纤维蛋白和先进血小板富纤维蛋白。从同一供体的血液中制备相应的膜，探讨了生长因子的释放、宏观尺寸、细胞含量和机械性能。此外，还通过形态学和电子显微镜扫描评估了时间（采血-离心和离心-膜制备）的影响。在生长因子释放或细胞含量，或凝块/膜尺寸方面，三种 PRF 改良型之间没有观察到统计学上的显著差异。当使用相同的 g 力时，两种离心机之间的差异可以忽略不计。然而，较低的 g 力会降低膜的拉伸强度。制备过程中的时间安排有重大影响。RCF 的适应仅对 PRF 膜的最终特性产生最小的影响。
