牛冠状病毒感染诱导的细胞融合分析。
Analysis of cell fusion induced by bovine coronavirus infection.
作者信息
Payne H R, Storz J
机构信息
Department of Veterinary Microbiology and Parasitology, Louisiana State University, Baton Rouge.
出版信息
Arch Virol. 1988;103(1-2):27-33. doi: 10.1007/BF01319806.
Abstract
Polykaryon formation in bovine fetal spleen (BFS) cells infected with bovine coronavirus L9 occurred only in media supplemented with trypsin. A single 1 to 2 h trypsin treatment 10 h and later after infection induced formation of polykaryons. Trypsin treatment at pH 7.5 and 8.0 induced polykaryons while treatments at lower or higher pH levels did not. Cell fusion activity was partially suppressed by the presence of antibody.
摘要
感染牛冠状病毒L9的牛胎儿脾脏（BFS）细胞中的多核体形成仅在添加胰蛋白酶的培养基中发生。在感染后10小时及更晚进行单次1至2小时的胰蛋白酶处理可诱导多核体形成。在pH 7.5和8.0条件下的胰蛋白酶处理可诱导多核体形成，而在较低或较高pH水平下的处理则不能。抗体的存在可部分抑制细胞融合活性。
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本文引用的文献
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