低pH诱导感染胡宁病毒的Vero细胞融合。
Low-pH-induced fusion of Vero cells infected with Junin virus.
作者信息
Castilla V, Mersich S E
机构信息
Laboratorio de Virologia, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
出版信息
Arch Virol. 1996;141(7):1307-17. doi: 10.1007/BF01718832.
Abstract
Junin virus (JV) infected Vero cells were used to investigate virus capacity to induce cell-cell fusion. Polykaryocyte formation due to JV was found to be pH and temperature-dependent. A reduced fusion activity was detected on BHK-21 cells. Different JV-strains exhibited a similar extent and pH dependence of their fusion activity. Neutralizing antibodies against the main viral glycoprotein (GP38) inhibited syncytium production and GP38 conformational changes in response to acid treatment were detected by an immunoprecipitation assay.
摘要
用胡宁病毒（JV）感染的Vero细胞来研究病毒诱导细胞-细胞融合的能力。发现由JV引起的多核细胞形成与pH值和温度有关。在BHK-21细胞上检测到融合活性降低。不同的JV毒株表现出相似程度的融合活性及其对pH值的依赖性。针对主要病毒糖蛋白（GP38）的中和抗体抑制了多核体的产生，并且通过免疫沉淀试验检测到了GP38在酸处理后的构象变化。
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