肠杆菌科中转录核糖核酸和顺反子5S核糖核酸的保守性
Conservation of transfer ribonucleic acid and 5S ribonucleic acid cistrons in Enterobacteriaceae.
作者信息
Brenner D J, Fanning G R, Steigerwalt A G, Sodd M A, Doctor B P
出版信息
J Bacteriol. 1977 Mar;129(3):1435-9. doi: 10.1128/jb.129.3.1435-1439.1977.
Abstract
The genes for tranfer ribonucleic acid (tDNA) and 5S ribonucleic acid (5SDNA) were isolated from the total deoxyribonucleic acid (DNA) of Escherichia coli. The relatedness of tDNA and 5S from E. coli and other species of Enterobacteriaceae was determined by reassociation of the isolated genes labeled with 32PO4 to unlabeled, unfractionated DNA. Double-stranded DNA was separated from unreacted DNA by hydroxyapatite chromatography. Thermal elution profiles were done to determine the amount of unpaired bases present in related DNA sequences. Relative to total DNA, both 5S DNA and tDNA were highly conserved throughout the Enterobacteriaceae, including the genera Yersinia and Proteus.
摘要
从大肠杆菌的总脱氧核糖核酸（DNA）中分离出转移核糖核酸（tDNA）和5S核糖核酸（5SDNA）的基因。通过将用32PO4标记的分离基因与未标记的、未分级的DNA重新结合，来确定大肠杆菌和其他肠杆菌科物种的tDNA和5S的相关性。通过羟基磷灰石色谱法从未反应的DNA中分离出双链DNA。进行热洗脱图谱分析以确定相关DNA序列中存在的未配对碱基的量。相对于总DNA，5S DNA和tDNA在整个肠杆菌科中，包括耶尔森菌属和变形杆菌属，都高度保守。
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