细菌核糖体RNA顺反子的分离与特性分析。
Isolation and characterization of bacterial ribosomal RNA cistrons.
作者信息
Kohne D E
出版信息
Biophys J. 1968 Oct;8(10):1104-18. doi: 10.1016/S0006-3495(68)86542-7.
Abstract
The DNA sequences which code for ribosomal DNA have been isolated and purified. The technique used has general application for RNA:DNA hybridization studies and enables the isolation of any gene for which sufficient gene product can be obtained. Experiments with isolated ribosomal RNA cistrons demonstrated that (a) the majority of the ribosomal cistrons are similar to one another; (b) the cistrons which are similar to one another are virtually identical to one another; (c) ribosomal cistrons of different bacterial species are closely related to one another.
摘要
编码核糖体DNA的DNA序列已被分离和纯化。所使用的技术在RNA:DNA杂交研究中具有普遍应用价值，并且能够分离出任何可获得足够基因产物的基因。对分离出的核糖体RNA顺反子进行的实验表明：（a）大多数核糖体顺反子彼此相似；（b）彼此相似的顺反子实际上彼此相同；（c）不同细菌物种的核糖体顺反子彼此密切相关。
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