验证 Microreader™ 29Y Prime ID 系统在法医中的应用。
Validation of the Microreader™ 29Y Prime ID system for forensic use.
机构信息
Forensic Science Service of the Beijing Public Security Bureau, 100192, Beijing, China.
Taiyuan Public Security Bureau, 030001, Taiyuan, China.
出版信息
Forensic Sci Int. 2020 Apr;309:110147. doi: 10.1016/j.forsciint.2020.110147. Epub 2020 Jan 15.
Currently, Y-short tandem repeat loci (Y-STRs) have been increasingly used in the forensic field, particularly in investigations of sexual assault, determination of paternity and male lineage studies because of the characteristics of male-only and paternal inheritance. The Microreader™ 29Y Prime ID system is a 29-plex Y-STR genotyping system that amplifies 17 widely used commercial loci (DYS570, DYS546, DYS460, DYS458, DYS635, DYS533, DYS448, DYS627, DYS456, DYS576, DYS449, DYS437, DYS643, DYS518, DYF387S1 a/b, and a sexual locus Y GATA H4), European recommended 7 single-copy "minimal haplotypes" (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, and DYS385a/b) and 2 additional loci (DYS438 and DYS439) recommended by The Scientific Working Group on DNA Analysis Methods (SWGDAM). The Microreader™ 29Y Prime ID system was validated according to the guidelines of "Validation Guidelines for DNA Analysis Methods (2016)" described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based, sensitivity, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. This study indicates that the Microreader™ 29Y Prime ID system is a useful tool for forensic cases and Y-STR genotyping.
目前，Y 短串联重复序列（Y-STR）由于其仅雄性遗传和父系遗传的特点，已越来越多地应用于法医领域，特别是在性侵犯调查、亲子关系鉴定和男性谱系研究中。Microreader™ 29Y Prime ID 系统是一种 29 plex Y-STR 基因分型系统，可扩增 17 个广泛使用的商业位点（DYS570、DYS546、DYS460、DYS458、DYS635、DYS533、DYS448、DYS627、DYS456、DYS576、DYS449、DYS437、DYS643、DYS518、DYF387S1 a/b 和一个性位点 Y GATA H4）、欧洲推荐的 7 个单拷贝“最小单倍型”（DYS19、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393 和 DYS385a/b）和 2 个额外的位点（DYS438 和 DYS439）。Microreader™ 29Y Prime ID 系统是根据 DNA 分析方法科学工作组（SWGDAM）描述的“DNA 分析方法验证指南（2016）”的准则进行验证的，包括基于 PCR 的灵敏度、精密度和准确性评估、不稳定性、峰高比、抑制物、种特异性和 DNA 混合物研究。本研究表明，Microreader™ 29Y Prime ID 系统是法医案件和 Y-STR 基因分型的有用工具。
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