Microreader™ Y Prime Plus ID系统的发育验证：一种用于法医应用的先进Y-STR 38重体系。
Developmental validation of the Microreader™ Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications.
作者信息
Zhao Yi, Yao Xiaojuan, Li Yifan, Chen Chengjian, Guo Dan, Jiao Zhangping, Yang Zhipan, Yu Zailiang, Ma Wanshan, Zhang Qingxia
机构信息
Forensic Science Service of the Beijing Public Security Bureau, 100192 Beijing, China.
Forensic Science Service of the Xinzhou Public Security Bureau, 034000 Shanxi, China.
出版信息
Sci Justice. 2021 May;61(3):260-270. doi: 10.1016/j.scijus.2021.01.003. Epub 2021 Jan 30.
Y-STR is widely used in sexual assaults and familial searches of suspects. Here, we reported a novel 38-plex STR genotyping system designed for forensic applications. Microreader™ Y Prime Plus ID System (YPP) amplifies 38 loci in one reaction, including 29 loci from commonly used Yfiler® Plus PCR Amplification Kit & PowerPlex® Y23 System (DYS393, DYS570, DYS19, DYS392, DYS549, Y GATA H4, DYS460, DYS458, DYS481, DYS635, DYS448, DYS533, DYS449, DYS456, DYS389I, DYS390, DYS389Ⅱ, DYS438, DYS391, DYS439, DYS437, DYS385a/b, DYS643, DYS518, DYS576, DYF387S1a/b, and DYS627), 6 commonly used loci for the Y-STR database (DYS444, DYS447, DYS596, DYF404a/b, DYS527a/b, DYS557) and one Y-indel specific for the Chinese population. YPP is designed for different types of samples, such as blood card and swabs. In this work, YPP was validated following SWGDAM guidelines (2016) and guidelines from Ministry of Public Security of the People's Republic of China, including PCR-based, sensitivity, accuracy and precision, mixture, stability and inhibitor, and species specificity. The results indicate that the Microreader™ Y Prime Plus ID System is a powerful identification kit designed for forensic databases.
Y染色体短串联重复序列（Y-STR）广泛应用于性侵犯案件及嫌疑人的家族搜索。在此，我们报告了一种专为法医应用设计的新型38重STR基因分型系统。微读™ Y Prime Plus ID系统（YPP）在一个反应中扩增38个位点，包括来自常用的Yfiler® Plus PCR扩增试剂盒和PowerPlex® Y23系统的29个位点（DYS393、DYS570、DYS19、DYS392、DYS549、Y GATA H4、DYS460、DYS458、DYS481、DYS635、DYS448、DYS533、DYS449、DYS456、DYS389I、DYS390、DYS389Ⅱ、DYS438、DYS391、DYS439、DYS437、DYS385a/b、DYS643、DYS518、DYS出576、DYF387S1a/b和DYS627）、Y-STR数据库常用的6个位点（DYS444、DYS447、DYS596、DYF404a/b、DYS527a/b、DYS557）以及一个针对中国人群的Y染色体插入/缺失多态性位点。YPP适用于不同类型的样本，如血卡和拭子。在这项工作中，YPP按照美国法医DNA分析方法科学工作组（SWGDAM）指南（2016年）以及中华人民共和国公安部的指南进行了验证，包括基于聚合酶链反应（PCR）的验证、灵敏度、准确性和精密度、混合样本、稳定性和抑制剂以及物种特异性。结果表明，微读™ Y Prime Plus ID系统是一种为法医数据库设计的强大鉴定试剂盒。
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