蜱虫 Rhipicephalus haemaphysaloides 中的丝氨酸蛋白酶抑制剂：参与卵黄发生。
A serpin from the tick Rhipicephalus haemaphysaloides: Involvement in vitellogenesis.
机构信息
Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
出版信息
Vet Parasitol. 2020 Mar;279:109064. doi: 10.1016/j.vetpar.2020.109064. Epub 2020 Feb 27.
Tick serpins are involved in enzyme activity, food digestion, blood-feeding, immune response and anticoagulation. Little is known about the potential roles of serpins in tick reproduction. RHS8, a serpin from the tick Rhipicephalus haemaphysaloides, has an open reading frame 1212 bp long and encodes a protein that has 404 amino acids and a predicted molecular weight of 45 kDa. RHS8 exhibits 89.58 % amino acid identity with RmS15 in Rhipicephalus microplus. RHS8 was expressed primarily in larvae and nymphs. RHS8 mRNA expression in the ovaries, fat bodies and salivary glands were up-regulated from feeding to ovipositing ticks. RNAi results showed that RHS8 dsRNA-injected ticks had a lower body weight, longer feeding time, fewer eggs laid and lower egg hatchability. Tick reproduction, such as egg laying and hatching, was disrupted by RNAi. Compared with the control group, ovaries of the RHS8 interference group were light brown color, indicating a reduction in yolk granule accumulation. Western blot results showed that the expression of RHVg3 and RHVg4 proteins in ovaries was reduced in the RHS8 dsRNA-injected group. These results indicate that RHS8 is related to tick reproduction and its interference affects vitellogenesis.
蜱丝氨酸蛋白酶抑制剂参与酶活性、食物消化、吸血、免疫反应和抗凝。然而，关于蜱丝氨酸蛋白酶抑制剂在蜱繁殖中的潜在作用，人们知之甚少。来自硬蜱 Rhipicephalus haemaphysaloides 的丝氨酸蛋白酶抑制剂 RHS8 具有 1212bp 的开放阅读框，编码一个含有 404 个氨基酸和预测分子量为 45kDa 的蛋白质。RHS8 与 Rhipicephalus microplus 中的 RmS15 具有 89.58%的氨基酸同一性。RHS8 主要在幼虫和若虫中表达。从吸血到产卵的过程中，RHS8 在卵巢、脂肪体和唾液腺中的 mRNA 表达均上调。RNAi 结果表明，注射 RHS8 dsRNA 的蜱虫体重降低、吸血时间延长、产卵量减少且孵化率降低。RNAi 干扰了蜱的繁殖，如产卵和孵化。与对照组相比，RHS8 干扰组的卵巢呈浅棕色，表明卵黄颗粒积累减少。Western blot 结果表明，在 RHS8 dsRNA 注射组中，卵巢中 RHVg3 和 RHVg4 蛋白的表达减少。这些结果表明，RHS8 与蜱的繁殖有关，其干扰影响卵黄发生。
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