Department of Chemical Engineering, College of Engineering, Qatar University, P.O. Box 2713, Doha, Qatar.
Sci Total Environ. 2020 Jun 10;720:137594. doi: 10.1016/j.scitotenv.2020.137594. Epub 2020 Feb 26.
The optimum growth (μ), CO bio-fixation (R) rates and the energy ratio (ER) of microalgae Chlorella vulgaris (C.v) were identified using central composite design statistical approach (CCD-SA). μ and R parameters including temperature of photobioreactor (T), concentration of CO (C ), nutrients (carbon, nitrogen and phosphorus), gas flow rate (Q), initial inoculum concentration (IN) and the solar light intensity (I) were considered. Results revealed mild operational conditions in the range 20-25 °C, C of 2.5-20% (v/v), Q of 0.5-0.8 vvm and I of 50-200 μE/m·s would generate considerable μ and R. The highest μ and R with a significant ER of 19.5 were generated under CCD-SA optimized parameters of T = 25 °C, C = 20%, Q = 0.5 ± 0.05 (Std. Dev. = 0.04) vvm, total inorganic nitrogen (TN) = 19 ± 2 (Std. Dev. = 0.1) mg-N/L, Total phosphorous = 7 ± 1 (Std. Dev. = 0.7) mg-P/L, COD = 20 ± 2 (Std. Dev. = 0.5) mg-COD/L, IN = 0.52 ± 0.01 (Std. Dev. = 0.05) mg/L and I = 150 ± 2(Std. Dev. = 0.6) μE/ms). Microalgae technology can be considered as a promising technology for CO bio-fixation in a large scale with a sustainable value of the produced biomass for biofuel production.
采用中心复合设计统计方法（CCD-SA）确定了普通小球藻（C.v）的最佳生长（μ）、CO 生物固定（R）速率和能量比（ER）。μ和 R 参数包括光生物反应器温度（T）、CO 浓度（C）、营养物质（碳、氮和磷）、气体流量（Q）、初始接种浓度（IN）和太阳光照强度（I）。结果表明，在 20-25°C、2.5-20%（v/v）的 C、0.5-0.8 vvm 的 Q 和 50-200 μE/m·s 的 I 范围内，可以产生相当高的μ和 R，操作条件较为温和。在 CCD-SA 优化的 T=25°C、C=20%、Q=0.5±0.05（标准偏差=0.04）vvm、总无机氮（TN）=19±2（标准偏差=0.1）mg-N/L、总磷（TP）=7±1（标准偏差=0.7）mg-P/L、COD=20±2（标准偏差=0.5）mg-COD/L、IN=0.52±0.01（标准偏差=0.05）mg/L 和 I=150±2（标准偏差=0.6）μE/ms 等优化参数下，产生了最高的μ和 R，以及显著的 ER 为 19.5。微藻技术可以被认为是一种很有前途的 CO 生物固定技术，可以在大规模生产中应用，并且具有可持续的生物量价值，可用于生物燃料生产。
