Wang Bo, Li Zhen, Li Heqiong, Luo Hailing, Blair Hugh T, Jian Luyang, Diao Zhicheng
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Sheep Research Group, School of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand.
Animals (Basel). 2020 Mar 4;10(3):433. doi: 10.3390/ani10030433.
The objective of the present study was to investigate the dynamic change of serum parameters and milk composition by dietary FA supplementation with ewes with different litter size from mating to lambing. The ewes were divided into six treatments (TW-CON, TW-F16, TW-F32, TR-CON, TR-F16, TR-F32) according to dietary FA levels (control, CON; 16 or 32 mg·kg rumen-protect-FA supplementation, F16 and F32) and litter size (twin born, TW; and triplet born, TR). In serum, the concentration of folate increased linearly with dietary FA supplementation ( < 0.05), regardless of the litter size, they showed a quadratic response to gestation progression ( < 0.05). With dietary FA addition, IGFI-I levels significant increased from late gestation to after lambing ( < 0.05), and linearly increased immunoglobulin during the perinatal period ( < 0.05). In colostrum and milk at d 15, the content of folate, lactoferrin, and IgG were affected positively by FA supplementation ( < 0.05). IgG was higher in the TW group than TR in colostrum ( < 0.05), and lactoferrin in TW was lower than TR in milk of d 15 ( < 0.05). FA supplementation increased protein content in colostrum ( < 0.05), while it had no effect on the fat, lactose, and BUN of colostrum and milk of d 15 ( > 0.05). These results suggest that FA supplementation during gestation could regulate maternal blood metabolism and contribute to milk immune composition.
本研究的目的是调查在配种至产羔期间，对不同产羔数的母羊进行日粮脂肪酸（FA）补充后，血清参数和乳汁成分的动态变化。根据日粮FA水平（对照组，CON；补充16或32 mg·kg瘤胃保护FA，F16和F32）和产羔数（双羔，TW；三羔，TR），将母羊分为六个处理组（TW-CON、TW-F16、TW-F32、TR-CON、TR-F16、TR-F32）。在血清中，叶酸浓度随日粮FA补充呈线性增加（P<0.05），无论产羔数如何，它们对妊娠进程呈二次反应（P<0.05）。添加日粮FA后，胰岛素样生长因子I（IGFI-I）水平从妊娠后期到产羔后显著增加（P<0.05），围产期免疫球蛋白呈线性增加（P<0.05）。在第15天的初乳和乳汁中，叶酸、乳铁蛋白和IgG的含量受FA补充的正向影响（P<0.05）。初乳中TW组的IgG高于TR组（P<0.05），第15天乳汁中TW组的乳铁蛋白低于TR组（P<0.05）。FA补充增加了初乳中的蛋白质含量（P<0.05），而对第15天初乳和乳汁中的脂肪、乳糖和尿素氮没有影响（P>0.05）。这些结果表明，妊娠期补充FA可调节母体血液代谢并有助于乳汁免疫成分的形成。
