邻苯二甲酸二（2-乙基己基）酯处理的卵巢细胞中的基因差异表达。
Differential Expression of Genes in Ovarian Cells Treated with Di-2-Ethylhexyl Phthalate.
机构信息
Department of Biomedical Science, CHA University, Gyeonggi-Do 13488, Korea.
Department of Obstetrics and Gynecology, Korea University Anam Hospital, Korea University College of Medicine, Seoul 02841, Korea.
出版信息
Int J Mol Sci. 2020 Mar 4;21(5):1755. doi: 10.3390/ijms21051755.
Premature ovarian failure (POF) is defined as loss of ovarian function in women less than 40 years of age. The causes of POF are diverse and include environmental factors. Di-2-ethylhexyl phthalate (DEHP) is one factor that may cause POF. The ubiquitin-proteasome system maintains intracellular balance by promoting or inhibiting protein degradation. To investigate the differential expressions of () genes in patients with POF, we developed two in vitro POF models by treating A2780 or OVCAR5 with DEHP. Using these models, a multiplex RT-PCR system for genes was applied to identify biomarkers by comparing expression patterns and mRNA levels; multiplex RT-PCR results were validated by qRT-PCR and Western blotting analyses. Observed differential expression levels of several genes including , , , , and in A2780 and OVCAR5 cells at the mRNA and protein levels suggest that they should be investigated as potential biomarkers of POF.
卵巢早衰（POF）是指 40 岁以下女性卵巢功能丧失。POF 的病因多种多样，包括环境因素。邻苯二甲酸二（2-乙基己基）酯（DEHP）是可能导致 POF 的因素之一。泛素-蛋白酶体系统通过促进或抑制蛋白质降解来维持细胞内平衡。为了研究 POF 患者 () 基因的差异表达，我们通过用 DEHP 处理 A2780 或 OVCAR5 建立了两种体外 POF 模型。使用这些模型，通过比较表达模式和 () mRNA 水平，应用多重 RT-PCR 系统鉴定生物标志物；通过 qRT-PCR 和 Western blot 分析验证了多重 RT-PCR 结果。在 A2780 和 OVCAR5 细胞中观察到几个 () 基因，包括 、 、 、 和 ，在 mRNA 和蛋白质水平上的差异表达水平表明它们应该作为 POF 的潜在生物标志物进行研究。
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