Cui Zhaohui, Wang Luyang, Wang Yuexin, Li Juan, Wang Rongjun, Sun Mingfei, Zhang Longxian
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Microorganisms. 2020 Mar 4;8(3):363. doi: 10.3390/microorganisms8030363.
is a zoonotic intracellular protozoan responsible for the diarrheal illness cryptosporidiosis in humans and animals. Although a number of zoite surface proteins are known to be expressed during, and believed to be involved in, attachment and invasion of host cells, the molecular mechanisms by which invades the host epithelial cells are not well understood. In the present study, we investigated the gene expression patterns, protein localization in developmental stages in culture, and in vitro neutralization characteristics of Cpgp40/15 and Cpgp40. Indirect immunofluorescence assay showed that Cpgp40/15 is associated with the parasitophorous vacuole membrane (PVM) during intracellular development. Both anti-gp40/15 and anti-gp40 antibodies demonstrated the ability to neutralize infection in vitro. Further studies are needed to fully understand the specific role and functional mechanism of Cpgp40/15 (or gp40/15 complex) in the invasion of the host or in the PVM and to determine the feasibility of gp40/15 as a vaccine candidate for cryptosporidiosis in vivo.
是一种人畜共患的细胞内原生动物，可导致人类和动物患腹泻性疾病隐孢子虫病。虽然已知许多子孢子表面蛋白在宿主细胞的附着和入侵过程中表达，并被认为参与其中，但关于其入侵宿主上皮细胞的分子机制尚不清楚。在本研究中，我们研究了Cpgp40/15和Cpgp40的基因表达模式、在培养发育阶段的蛋白质定位以及体外中和特性。间接免疫荧光分析表明，Cpgp40/15在细胞内发育过程中与寄生泡膜（PVM）相关。抗gp40/15和抗gp40抗体均显示出在体外中和感染的能力。需要进一步研究以充分了解Cpgp40/15（或gp40/15复合物）在宿主入侵或PVM中的具体作用和功能机制，并确定gp40/15作为隐孢子虫病体内疫苗候选物的可行性。
