Ohashi K, Masaki T, Maruyama K
J Biochem. 1977 Jan;81(1):237-42. doi: 10.1093/oxfordjournals.jbchem.a131440.
Rabbit antiserum raised agains I-protein was used for immunofluorescent staining of chicken myofibrils. The FITC-conjugated anti-I-protein antibody stained A-band regions except at their middle regions. According to the conditions used, the myofibrils stained by their fluorescent antibody showed slightly different patterns, i.e., the nonstained regions in the center of the A-bands were wider. On fixing with glutaraldehyde, myofibrils were stained in the A-band regions except at their middle regions. Therefore, I-protein may be localized at A-bands except for the center.
用针对I蛋白产生的兔抗血清对鸡肌原纤维进行免疫荧光染色。异硫氰酸荧光素（FITC）偶联的抗I蛋白抗体对A带区域进行染色，但A带中间区域除外。根据所用条件，用荧光抗体染色的肌原纤维呈现出略有不同的模式，即A带中央的未染色区域更宽。用戊二醛固定后，肌原纤维在A带区域（中间区域除外）被染色。因此，I蛋白可能定位于除中央以外的A带区域。
