Ohashi K, Tsuneoka M, Maruyama K
J Biochem. 1985 May;97(5):1323-8. doi: 10.1093/oxfordjournals.jbchem.a135184.
FITC-labeled antibodies raised against chicken myofibrillar I-protein stained chicken myofibrils, which were fixed with formalin immediately after being cut from the sacrificed chicken breast muscle, at the junctional region of A-bands and I-bands. On the other hand, the antibodies stained the glycerinated myofibrils at the region around Z-bands. Aged glycerinated myofibrils stored in a cold room became stained with the same antibodies at the M-line and the A-band region except for the H-zone and the Z-band. I-Protein, which was originally localized at the A-I junctions, moved to the region around Z-bands and A-bands during the process of preparing myofibrils, paralleling the deterioration of myofibrils. Although I-protein is easily released from its original position, it is not a cytoplasmic protein of muscle but an intrinsic myofibrillar component, because immunoblotting tests showed that I-protein is contained in the myofibrillar fraction and not in the muscular cytoplasmic fraction.
用抗鸡肌原纤维I蛋白产生的异硫氰酸荧光素标记抗体，对从处死的鸡胸肌切下后立即用福尔马林固定的鸡肌原纤维，在A带和I带的交界区域进行染色。另一方面，这些抗体在Z带周围区域对甘油处理的肌原纤维进行染色。保存在冷藏室中的老化甘油处理肌原纤维，除H区和Z带外，在M线和A带区域被相同的抗体染色。原本定位于A-I交界处的I蛋白，在制备肌原纤维的过程中，随着肌原纤维的降解，转移到Z带和A带周围区域。尽管I蛋白很容易从其原始位置释放，但它不是肌肉的细胞质蛋白，而是肌原纤维的固有成分，因为免疫印迹试验表明I蛋白存在于肌原纤维部分，而不存在于肌肉细胞质部分。
