Chemistry Institute, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, 21941-909, Brazil.
Center for Food Analysis (NAL-LADETEC), Rio de Janeiro, 21941-598, Brazil.
Sci Rep. 2020 Mar 6;10(1):4243. doi: 10.1038/s41598-020-61293-8.
This study investigated the physicochemical, instrumental and bacterial parameters of tilapia fillets subjected to oxygen-scavenger packaging, alone or in combination with UV-C radiation at two doses (0.102 and 0.301 J/cm), stored at 4 ± 1 °C for 23 days. The oxygen scavenger, both UV-C doses, and the oxygen scavenger combined with UV-C, independently of the dose, extended the shelf life in 5, 6 and 7 days, respectively, by decreasing the bacterial growth rate and the formation of degradation compounds (e.g., TVB-N and ammonia). Oxygen-scavenger packaging, alone or in combination with UV-C at 0.102 J/cm and 0.301 J/cm showed lower amounts of free amino acids (FAA; 34.39, 34.49 and 34.50 mg L-lysine/kg fish tissue, 3.63, 3.57 and 3.61 mg L- ornithine/kg fish tissue, 27.52, 27.63 and 27.67 mg L-arginine/kg fish tissue), biogenic amines (BA; 3.81, 3.87 and 3.89 mg cadaverine/kg fish tissue, 12.88, 12.91 and 12.86 mg putrescine/kg fish tissue, 2.41, 2.44 and 2.47 mg spermidine/kg fish tissue), redness (2.53, 2.55 and 2.59), yellowness (6.65, 6.69 and 6.72), lipid oxidation (1.52, 1.53 and 1.58 mg malondialdehyde/kg fish tissue) and protein oxidation (5.06, 5.11 and 5.18 nmol carbonyls/mg protein), with higher hardness (3273.41, 2652.98 and 2687.57 g) than control (air packaging; 41.97 mg L-lysine/kg fish tissue, 4.83 mg L- ornithine/kg fish tissue, 37.33 mg L-arginine/kg fish tissue, 4.82 mg cadaverine/kg fish tissue, 16.56 mg putrescine/kg fish tissue, 3.21 mg spermidine/kg fish tissue, 4.26 of redness, 8.17 of yellowness, 2.88 mg malondialdehyde/kg fish tissue, 9.44 nmol carbonyls/mg protein and 2092.58 g of hardness), respectively, on day 13 of storage when the control fillets were unfit for consumption (7 log CFU/g) (p < 0.05). However, in the same day of storage, both UV-C doses had similar values for BA (p > 0.05), higher amounts of FAA (44.28 and 44.13 mg L-lysine/kg fish tissue, 5.16 and 5.12 mg L- ornithine/kg fish tissue, 40.20 and 40.28 mg L-arginine/kg fish tissue), redness (4.86 and 5.33), yellowness (9.32 and 10.01), lipid oxidation (3.09 and 3.52 mg malondialdehyde/kg fish tissue) and protein oxidation (10.27 and 11.93 nmol carbonyls/mg protein), as well as lower hardness (1877.54 and 1767.39 g), respectively, than control fillets (p < 0.05). The combined preservation methods were the most effective in extending the shelf life and prolonging the physicochemical quality of the refrigerated tilapia fillets and the O scavenger proved to be a potential alternative to prevent the negative changes induced by both UV-C doses.
本研究调查了罗非鱼片在单独或与 2 种剂量（0.102 和 0.301 J/cm）的紫外线-C（UV-C）辐射结合使用的情况下，经吸氧剂包装后在 4 ± 1°C 下储存 23 天的物理化学、仪器和细菌参数。吸氧剂、两种紫外线-C 剂量以及紫外线-C 与吸氧剂的组合，独立于剂量，分别延长了保质期 5、6 和 7 天，通过降低细菌生长速度和降解化合物的形成（例如 TVB-N 和氨）。单独的吸氧剂包装，或与 0.102 J/cm 和 0.301 J/cm 的紫外线-C 结合使用，显示出较低的游离氨基酸（FAA；34.39、34.49 和 34.50 mg L-赖氨酸/鱼组织 kg、3.63、3.57 和 3.61 mg L-鸟氨酸/鱼组织 kg、27.52、27.63 和 27.67 mg L-精氨酸/鱼组织 kg）、生物胺（BA；3.81、3.87 和 3.89 mg 腐胺/鱼组织 kg、12.88、12.91 和 12.86 mg 尸胺/鱼组织 kg、2.41、2.44 和 2.47 mg 亚精胺/鱼组织 kg）、红色度（2.53、2.55 和 2.59）、黄色度（6.65、6.69 和 6.72）、脂质氧化（1.52、1.53 和 1.58 mg 丙二醛/鱼组织 kg）和蛋白质氧化（5.06、5.11 和 5.18 nmol 羰基/mg 蛋白），与对照组（空气包装；41.97 mg L-赖氨酸/鱼组织 kg、4.83 mg L-鸟氨酸/鱼组织 kg、37.33 mg L-精氨酸/鱼组织 kg、4.82 mg 腐胺/鱼组织 kg、16.56 mg 尸胺/鱼组织 kg、3.21 mg 亚精胺/鱼组织 kg、4.26 的红色度、8.17 的黄色度、2.88 mg 丙二醛/鱼组织 kg、9.44 nmol 羰基/mg 蛋白和 2092.58 g 的硬度）相比，具有更高的硬度（3273.41、2652.98 和 2687.57 g），分别在第 13 天的储存时，对照组鱼片不适合食用（7 log CFU/g）（p < 0.05）。然而，在同一天的储存中，两种紫外线-C 剂量的 BA 值相似（p > 0.05），FAA 含量较高（44.28 和 44.13 mg L-赖氨酸/鱼组织 kg、5.16 和 5.12 mg L-鸟氨酸/鱼组织 kg、40.20 和 40.28 mg L-精氨酸/鱼组织 kg）、红色度（4.86 和 5.33）、黄色度（9.32 和 10.01）、脂质氧化（3.09 和 3.52 mg 丙二醛/鱼组织 kg）和蛋白质氧化（10.27 和 11.93 nmol 羰基/mg 蛋白），以及较低的硬度（1877.54 和 1767.39 g），分别与对照组鱼片（p < 0.05）相比。联合保存方法在延长冷藏罗非鱼片的货架寿命和延长其理化质量方面最为有效，吸氧剂被证明是一种防止两种紫外线-C 剂量引起的负面变化的潜在替代品。
