Department of Obstetrics and Gynecology, Inha University College of Medicine, Inha University Hospital, 27, Inhang-Ro, Jung-Gu, Incheon, 22332, South Korea.
Department of Pathology, Inha University College of Medicine, Inha University Hospital, Incheon, South Korea.
Arch Gynecol Obstet. 2020 Apr;301(4):949-954. doi: 10.1007/s00404-020-05476-8. Epub 2020 Mar 6.
Progesterone receptor membrane component 1 (PGRMC1) have anti-inflammatory and anti-apoptotic properties. This study aimed to determine the expression of PGRMC1 in fetal membranes among women with preterm labor (PTL), preterm premature rupture of membranes (PPROM), and acute histologic chorioamnionitis (HCA) during preterm birth.
Full thickness fetal membranes were obtained from women with gestational age-matched (32-34 weeks of gestational age), and categorized as PTL without HCA (PTL, n = 10), PPROM without HCA (PPROM, n = 10), PPROM with HCA (HCA, n = 10), and term without labor and HCA (term birth (TB), n = 9). The expression of PGRMC1 was assessed using western blot and Immunohistochemistry (IHC). As CD14 is a component of the innate immune system during inflammation, CD14 was used as inflammatory indicator. Nonparametric statistics were used for analysis.
PGRMC1 expression for all of preterm birth was lower than in TB (P = 0.01). In HCA, PGRMC1 expression was significantly decreased compared to that in PTL and PPROM (P = 0.006. P = 0.001, respectively). PGRMC1 expression in PPROM was higher than that in PTL (P = 0.002). There was a negative correlation between PGRMC1 and CD 14/β-actin ratio (r = - 0.518; P = 0.002). IHC showed that PGRMC1 was predominant in the cytoplasm of cells, these results were consistent with those of the western blot analysis.
Preterm birth with PTL, PPROM, and especially HCA is associated with a decreased PGRMC1 in fetal membranes and inversely associated with increased CD 14.
孕激素受体膜组份 1（PGRMC1）具有抗炎和抗凋亡作用。本研究旨在确定早产（PTL）、早产胎膜早破（PPROM）和早产急性组织学绒毛膜羊膜炎（HCA）患者胎膜中 PGRMC1 的表达。
收集胎龄匹配（32-34 周妊娠）的 PTL 无 HCA（PTL，n=10）、PPROM 无 HCA（PPROM，n=10）、PPROM 合并 HCA（HCA，n=10）和足月无临产和 HCA（足月分娩（TB），n=9）患者的完整胎膜。采用 Western blot 和免疫组化（IHC）检测 PGRMC1 的表达。CD14 是炎症过程中固有免疫系统的组成部分，因此将 CD14 作为炎症标志物。采用非参数统计分析进行分析。
所有早产患者的 PGRMC1 表达均低于 TB（P=0.01）。在 HCA 中，PGRMC1 的表达明显低于 PTL 和 PPROM（P=0.006，P=0.001）。PPROM 中 PGRMC1 的表达高于 PTL（P=0.002）。PGRMC1 与 CD14/β-肌动蛋白比值呈负相关（r=-0.518；P=0.002）。IHC 显示 PGRMC1 主要位于细胞的细胞质中，与 Western blot 分析结果一致。
PTL、PPROM 尤其是 HCA 与胎膜中 PGRMC1 减少相关，与 CD14 增加相关。
