Stouthandel Michael E J, Vanhove Christian, Devriendt Wouter, De Bock Sander, Debbaut Charlotte, Vangestel Carl, Van Hoof Tom
Department of Human Structure and Repair, Ghent University, Radiotherapy Park, Entrance 98, C. Heymanslaan 10, 9000, Ghent, Belgium.
Infinity Lab, Ghent University, Building P8, C. Heymanslaan 10, 9000, Ghent, Belgium.
Anat Sci Int. 2020 Jun;95(3):399-407. doi: 10.1007/s12565-020-00535-1. Epub 2020 Mar 6.
The aim of this study was to determine the effect of Thiel embalming on the biomechanical properties of nerve tissue, to validate the use of Thiel embalmed bodies as a reliable model system for obtaining biomechanical data to supplement neurodynamic models, for anesthesiological and neurosurgical training and for future preclinical test set-ups involving nerve tissue. Upon the arrival of a body at the anatomy department, a fresh median nerve was harvested, the harvest site was sutured and following the Thiel embalming procedure the Thiel embalmed median nerve of the opposing wrist was harvested. Micro CT was performed to establish the cross-sectional area and biomechanical tensile testing was performed to compare the Young's modulus/elasticity of fresh frozen and Thiel embalmed nerves. Thiel embalming did not cause a significant difference in elasticity when comparing Thiel embalmed and fresh frozen specimens. A correlation was found between the cross-sectional area of Thiel embalmed nerve specimens and their Young's modulus. Thiel embalming does not significantly alter the elasticity of nerve tissue compared to fresh frozen nerve tissue. Similar shapes were observed when comparing the stress/strain curves of both specimen types. This indicates that Thiel embalmed nerve tissue is a viable alternative for using fresh frozen specimens when investigating biomechanical principles/mechanisms. Some specimens showed a reversed trend in Young's modulus that could be related to slight differences in embalming outcome, so caution is advised when Thiel embalmed specimens are used to obtain raw numerical data for direct application in the clinic.
本研究的目的是确定蒂尔防腐处理对神经组织生物力学特性的影响，验证使用经蒂尔防腐处理的尸体作为可靠的模型系统，以获取生物力学数据，补充神经动力学模型，用于麻醉学和神经外科培训以及未来涉及神经组织的临床前测试设置。尸体运抵解剖学系后，采集一条新鲜的正中神经，缝合采集部位，在进行蒂尔防腐处理后，采集对侧手腕经蒂尔防腐处理的正中神经。进行微型计算机断层扫描以确定横截面积，并进行生物力学拉伸试验，以比较新鲜冷冻和经蒂尔防腐处理的神经的杨氏模量/弹性。在比较经蒂尔防腐处理的标本和新鲜冷冻的标本时，蒂尔防腐处理并未导致弹性出现显著差异。发现经蒂尔防腐处理的神经标本的横截面积与其杨氏模量之间存在相关性。与新鲜冷冻的神经组织相比，蒂尔防腐处理不会显著改变神经组织的弹性。比较两种标本类型的应力/应变曲线时，观察到相似的形状。这表明在研究生物力学原理/机制时，经蒂尔防腐处理的神经组织是使用新鲜冷冻标本的可行替代方案。一些标本的杨氏模量呈现相反趋势，这可能与防腐处理结果的细微差异有关，因此当使用经蒂尔防腐处理的标本获取直接应用于临床的原始数值数据时，建议谨慎使用。
