Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2020;2133:1-13. doi: 10.1007/978-1-0716-0434-2_1.
Expressed protein ligation is a method of protein semisynthesis and typically involves the reaction of recombinant protein C-terminal thioesters with N-cysteine containing synthetic peptides in a chemoselective ligation. The recombinant protein C-terminal thioesters are produced by exploiting the action of nature's inteins which are protein modules that catalyze protein splicing. This chapter discusses the basic principles of expressed protein ligation and recent advances and applications in this protein semisynthesis field. Comparative strengths and weaknesses of the method and future challenges are highlighted.
表达蛋白连接是一种蛋白质半合成方法，通常涉及重组蛋白 C 端硫酯与含有半胱氨酸的合成肽的化学选择性连接反应。重组蛋白 C 端硫酯是通过利用自然内含子的作用产生的，内含子是催化蛋白质剪接的蛋白质模块。本章讨论了表达蛋白连接的基本原理以及该蛋白质半合成领域的最新进展和应用。强调了该方法的比较优势和劣势以及未来的挑战。
