Klimov A N, Denisenko A D, Vinogradov A G, Nagornev V A, Pivovarova Y I, Sitnikova O D, Pleskov V M
Department of Biochemistry, Institute for Experimental Medicine of the USSR Academy of Medical Sciences, Leningrad.
Atherosclerosis. 1988 Nov;74(1-2):41-6. doi: 10.1016/0021-9150(88)90189-x.
The interaction of mouse peritoneal and human pericardial macrophages with lipoprotein (LP)-antibody (Ab) immune complex isolated from the serum of ischemic heart disease (IHD) patients has been studied. It is shown that the Ab of the autoimmune complex belongs to IgG class, and the antigen is the LP with d less than 1.063 g/ml. Incubation of mouse peritoneal macrophages with such complex led to the activation of [14C]oleic acid incorporation into cholesteryl esters by 2.5-2.8-fold, in comparison with the experiments where macrophages were incubated with free apoprotein (apo) B-containing LP isolated from the same serum. Incubation of human pericardial macrophages with autologous LP-Ab immune complex led to the transformation of macrophages into foam cells. These data lead to the conclusion that formation of LP-Ab autoimmune complexes may play an important role in the formation of atherosclerotic lesions of the arteries.
对小鼠腹腔巨噬细胞和人心包巨噬细胞与从缺血性心脏病（IHD）患者血清中分离出的脂蛋白（LP）-抗体（Ab）免疫复合物之间的相互作用进行了研究。结果表明，自身免疫复合物的抗体属于IgG类，抗原是密度小于1.063 g/ml的LP。与巨噬细胞与从同一血清中分离出的游离含载脂蛋白（apo）B的LP孵育的实验相比，用这种复合物孵育小鼠腹腔巨噬细胞导致[14C]油酸掺入胆固醇酯的活性提高了2.5至2.8倍。用人心包巨噬细胞与自体LP-Ab免疫复合物孵育导致巨噬细胞转变为泡沫细胞。这些数据得出结论，LP-Ab自身免疫复合物的形成可能在动脉粥样硬化病变的形成中起重要作用。
