Klimov A N, Denisenko A D, Popov A V, Nagornev V A, Pleskov V M, Vinogradov A G, Denisenko T V, Kheifes G M, Kuznetzov A S
Atherosclerosis. 1985 Dec;58(1-3):1-15. doi: 10.1016/0021-9150(85)90051-6.
Immune complexes consisting of human [125I]LDL or [125I]VLDL and anti-apo B IgG were prepared in vitro. After intravenous administration of these complexes or free LDL to normal rabbits the elimination rate for complexes was 2-3-fold higher than for free lipoproteins. The liver/spleen radioactivity ratio after administration of immune complexes was 34% less than after administration of free lipoproteins. Investigations carried out on the cell cultures have shown that during 4-h incubation the uptake of [125I]LDL-anti-apo B IgG complex by human lung fibroblasts was lower than uptake of free [125I]LDL, whereas mouse peritoneal macrophages took up immune complex more actively than free LDL. During 3 days of incubation of macrophages with LDL-anti-apo B IgG the transformation of macrophages into foam cells was observed. This process was accompanied by almost a 60-fold increase of cholesteryl ester content in the cell. It is suggested that excessive uptake of lipoprotein-antibody complexes by macrophages leading to formation of foam cells may play an important role in atherogenesis.
由人[125I]低密度脂蛋白（LDL）或[125I]极低密度脂蛋白（VLDL）与抗载脂蛋白B IgG组成的免疫复合物在体外制备。将这些复合物或游离LDL静脉注射给正常兔子后，复合物的清除率比游离脂蛋白高2至3倍。注射免疫复合物后肝脏/脾脏放射性比值比注射游离脂蛋白后低34%。在细胞培养上进行的研究表明，在4小时的孵育期间，人肺成纤维细胞对[125I]LDL - 抗载脂蛋白B IgG复合物的摄取低于对游离[125I]LDL的摄取，而小鼠腹腔巨噬细胞摄取免疫复合物比游离LDL更活跃。在用LDL - 抗载脂蛋白B IgG孵育巨噬细胞3天的过程中，观察到巨噬细胞转变为泡沫细胞。这个过程伴随着细胞内胆固醇酯含量几乎增加60倍。有人提出，巨噬细胞对脂蛋白 - 抗体复合物的过度摄取导致泡沫细胞形成，这可能在动脉粥样硬化形成中起重要作用。
