Research Institute for Integrative Regenerative Biomedical Engineering, Dongguk University, Goyang, Republic of Korea.
Bio Research Center, Lugen Sci Co, Bucheon, Republic of Korea.
J Orthop Res. 2020 Sep;38(9):1987-1995. doi: 10.1002/jor.24653. Epub 2020 Mar 16.
To define the functional role of Krüppel-like factor (KLF) 10 as a modulator of chondrocyte hypertrophy in developing skeleton, the developmental features in the long bone of KLF10 knockout (KO) mice were investigated and the mesenchymal stem cells (MSCs) from KLF10 KO mice were characterized regarding chondrogenesis and osteogenesis. Delayed long bone growth and delayed formation of primary ossification center were observed in an early embryonic stage in KLF10 KO mouse along with very low Indian hedgehog expression in epiphyseal plate. While the chondrogenic potential of mouse MSCs from KLF10 KO mice appeared normal or slight decreased, hypertrophy and osteogenesis were extensively suppressed. These findings suggest that KLF10 is a mediator of chondrocyte hypertrophy in developing skeleton, and that suppression of KLF10 may be employed as a new strategy for preventing hypertrophy in cartilage regeneration using MSCs.
为了明确 Krüppel 样因子 10（KLF10）在发育中骨骼的软骨细胞肥大中的功能作用，研究了 KLF10 敲除（KO）小鼠长骨的发育特征，并对 KLF10 KO 小鼠的间充质干细胞（MSCs）进行了软骨生成和成骨特性分析。在 KLF10 KO 小鼠的早期胚胎阶段，观察到长骨生长延迟和初级骨化中心形成延迟，同时骺板中印度刺猬因子表达水平极低。尽管 KLF10 KO 小鼠的 MSC 的软骨生成潜能似乎正常或略有降低，但肥大和成骨被广泛抑制。这些发现表明，KLF10 是发育中骨骼中软骨细胞肥大的介质，抑制 KLF10 可能被用作使用 MSCs 进行软骨再生时防止肥大的新策略。
