微生物谷氨酰胺转氨酶对热变性蛋清蛋白酸诱导凝胶和乳化凝胶的机械性能和微观结构的影响。
Effect of microbial transglutaminase on the mechanical properties and microstructure of acid-induced gels and emulsion gels produced from thermal denatured egg white proteins.
机构信息
Department of Food Science, Engineering and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj Campus, Karaj, Iran.
Department of Food Science, Engineering and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj Campus, Karaj, Iran; Transfer Phenomena Laboratory (TPL), Controlled Release Center, Department of Food Science, Engineering and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj Campus, Karaj, Iran; Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
出版信息
Int J Biol Macromol. 2020 Jun 15;153:523-532. doi: 10.1016/j.ijbiomac.2020.03.008. Epub 2020 Mar 5.
In the current study, the impact of microbial transglutaminase (MTGase)-mediated crosslinking on the thermally denatured egg white protein (TD-EWP) made in alkaline pH in relation to their gelling properties of in gel and emulsion gel systems was investigated. Unlike native EWP that was not a good substrate for MTGase due to its compact structure, SDS-PAGE and gel solubility data showed the TD-EWP was sufficiently cross-linked by MTGase. The MTGase significantly (p < 0.05) increased the gel strength, fracture stress, fracture strain, and TPA parameters and decreased the frequency dependence of elastic modulus and stress dependence of creep compliance of TD-EWP gel and emulsion gel samples. The enhanced mechanical properties of the TE-EWP gelled samples treated with MTGase may be suitable for delivering sensitive compounds as well as in fabricating tailored scaffolds in tissue engineering and biomedical products.
在当前的研究中，研究了微生物转谷氨酰胺酶（MTGase）介导的交联对在碱性 pH 下制备的热变性蛋清蛋白（TD-EWP）的影响，以及它们在凝胶和乳状液凝胶系统中的胶凝特性。与由于其紧凑结构而不是 MTGase 良好底物的天然 EWP 不同，SDS-PAGE 和凝胶溶解度数据表明 TD-EWP 被 MTGase 充分交联。MTGase 显著（p<0.05）增加了 TD-EWP 凝胶和乳状液凝胶样品的凝胶强度、断裂应力、断裂应变和 TPA 参数，降低了弹性模量的频率依赖性和蠕变柔量的应力依赖性。用 MTGase 处理的 TE-EWP 凝胶样品增强的机械性能可能适合输送敏感化合物，以及在组织工程和生物医学产品中制造定制支架。
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