Chang Taehee, Jung Bong-Kwang, Shin Hyejoo, Hong Sooji, Lee Jeonggyu, Kim Deok-Gyu, Patarwut Laddawan, Sohn Woon-Mok, Chai Jong-Yil
Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Korean J Parasitol. 2020 Feb;58(1):87-91. doi: 10.3347/kjp.2020.58.1.87. Epub 2020 Feb 29.
Gymnophallid metacercariae found in the Manila clam Ruditapes philippinarum ('Banjirak' in Korean) from Gochang-gun, Jeollabuk-do, Korea were morphologically and molecularly confirmed to be Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974. The metacercariae were morphologically characterized by having a large oral sucker, small ventral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, which were all compatible with P. duboisi. Some of the metacercariae were experimentally fed to mice, and adult flukes were recovered at day 7 post-infection. The morphology of the adult flukes was basically the same as that of the metacercariae except for the presence of uterine eggs; the uterus was filled with up to 40 eggs. The nucleotide sequences (1,193 bp) from ITS regions (ITS1, 5.8S rDNA, and ITS2) of the metacercariae showed 99.7% identity with P. duboisi and 75.7% identity with Gymnophalloides seoi deposited in GenBank. These results confirmed the presence of P. duboisi metacercariae in the Manila clam R. philippinarum in an estuary region of Gochang-gun, Korea.
在韩国全罗北道高敞郡菲律宾蛤仔（韩国称为“Banjirak”）中发现的裸茎吸虫囊蚴，经形态学和分子学鉴定为杜氏小裸茎吸虫（Parvatrema duboisi）（多尔富斯，1923年）巴托利，1974年。这些囊蚴的形态特征为具有一个大的口吸盘、小的腹吸盘、腹吸盘前方一定距离处的生殖孔、无腹凹以及1个紧密或稍分叶的卵黄腺，这些都与杜氏小裸茎吸虫相符。部分囊蚴经实验性投喂给小鼠，感染后第7天回收了成虫吸虫。成虫吸虫的形态与囊蚴基本相同，只是存在子宫内的虫卵；子宫内充满了多达40个虫卵。囊蚴ITS区域（ITS1、5.8S rDNA和ITS2）的核苷酸序列（1193 bp）与杜氏小裸茎吸虫的序列一致性为99.7%，与GenBank中存放的西氏裸茎吸虫（Gymnophalloides seoi）的序列一致性为75.7%。这些结果证实了在韩国高敞郡河口地区的菲律宾蛤仔中存在杜氏小裸茎吸虫囊蚴。
