Veasey John Verrinder, Campaner Adriana Bittencourt, Lellis Rute Facchini
Dermatology Clinic, Santa Casa de Misericórdia de São Paulo, Vila Buarque, SP, Brazil.
Department of Ginecology and Obstetrics, Santa Casa de Misericórdia de São Paulo, Vila Buarque, SP, Brazil.
An Bras Dermatol. 2020 Mar-Apr;95(2):144-149. doi: 10.1016/j.abd.2019.06.007. Epub 2020 Feb 12.
Anogenital warts are the leading sexually transmitted infection in patients seeking care at specialized clinics. They may display a vast array of forms, according to the interaction of the virus with the host's immunity. Cellular immunity is the epithelium's main form of defense against the virus, involving an active participation of the Langerhans cells and pro-inflammatory cytokines such as TNF-α.
To assess the epithelial immune response of anogenital warts in males, according to the number of lesions presented.
This is a prospective, cross-sectional study carried out at the dermatology outpatient clinic in a tertiary hospital. We included male patients over 18 years of age without comorbidities who had anogenital condylomata and no previous treatments.In order to evaluate the local epithelial immunity, the lesions were quantified, then removed and employed in CD1a immunohistochemistry assays for assessing the morphometry and morphology of Langerhans cells; TNF-α; reaction was used for determining cytokine positivity in the epithelium.
48 patients were included in the study. There was no statistically significant difference as to the number of Langerhans cells, in their morphology, or the presence of TNF-α. However, patients presenting with more Langerhans cells in the lesions had cells with a star-like and dendritic morphology, whereas in those with a lower cell count had cells with a rounded morphology and no dendrites (p<0.001).
Small number of patients analyzed.
There was no difference in epithelial immunity between patients having few or many anogenital condyloma lesions as measured by the morphology and morphometry of Langerhans cells and TNF-α; positivity. Such an assessment employing immunity markers differing from the usual ones is expected to yield useful results.
肛门生殖器疣是在专科诊所就诊患者中最主要的性传播感染疾病。根据病毒与宿主免疫的相互作用，它们可能呈现出多种多样的形式。细胞免疫是上皮组织抵御病毒的主要防御形式，涉及朗格汉斯细胞和促炎细胞因子如肿瘤坏死因子-α的积极参与。
根据男性肛门生殖器疣的皮损数量评估其上皮免疫反应。
这是一项在三级医院皮肤科门诊进行的前瞻性横断面研究。我们纳入了年龄超过18岁、无合并症、患有肛门生殖器疣且未曾接受过治疗的男性患者。为了评估局部上皮免疫，对皮损进行量化，然后切除并用于CD1a免疫组化分析，以评估朗格汉斯细胞的形态计量学和形态；采用肿瘤坏死因子-α反应来确定上皮细胞因子阳性情况。
48名患者纳入研究。在朗格汉斯细胞数量、其形态或肿瘤坏死因子-α的存在方面，没有统计学上的显著差异。然而，皮损中朗格汉斯细胞较多的患者，其细胞呈星状和树突状形态，而细胞数量较少的患者其细胞呈圆形形态且无树突（p<0.001）。
分析的患者数量较少。
通过朗格汉斯细胞的形态计量学和形态以及肿瘤坏死因子-α阳性情况来衡量，肛门生殖器疣皮损少或多的患者之间上皮免疫没有差异。采用不同于常用免疫标志物的这种评估有望产生有用的结果。
