Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jun 5;233:118088. doi: 10.1016/j.saa.2020.118088. Epub 2020 Feb 12.
The surface-enhanced Raman spectroscopy (SERS) is a method known for its effectiveness in detecting and identifying microorganisms, that was employed to differentiate various bacterial strains both at genus and species level. In this work, we have examined five species belonging to Streptococcus genus, namely S. pneumoniae, S. suis, S. pseudopneumoniae, S. oralis, and S. mitis. Additionally, we conducted SERS experiments on ten S. pneumoniae strains, representing different capsular types. In all of cases we obtained unique SERS signals being spectroscopic fingerprints of bacterial strains tested. Moreover, the principal component analysis (PCA) was performed in order to prove that the spectra of all studied strains can be well separated into five (in case of streptococcal strains) or ten (in case of pneumococcal serotypes) groups. In both investigated situations, the separation at the level of 95% was achieved, proving that SERS-PCA-based method can be used for reliable and fast identification of different strains belonging to the Streptococcus genus, including encapsulated pneumococcal isolates.
表面增强拉曼光谱（SERS）是一种已知的在检测和识别微生物方面非常有效的方法，它被用于区分不同的细菌菌株，包括属和种水平。在这项工作中，我们研究了属于链球菌属的五个物种，即肺炎链球菌、猪链球菌、咽峡炎链球菌、口腔链球菌和缓症链球菌。此外，我们还对十种不同荚膜型的肺炎链球菌菌株进行了 SERS 实验。在所有情况下，我们都获得了独特的 SERS 信号，这些信号是测试菌株的光谱指纹。此外，还进行了主成分分析（PCA），以证明所有研究菌株的光谱可以很好地分为五个（在链球菌菌株的情况下）或十个（在肺炎球菌血清型的情况下）组。在这两种情况下，都达到了 95%的分离水平，证明了基于 SERS-PCA 的方法可用于可靠和快速鉴定属于链球菌属的不同菌株，包括囊膜性肺炎球菌分离株。
