Witkowska Evelin, Łasica Anna M, Niciński Krzysztof, Potempa Jan, Kamińska Agnieszka
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
ACS Sens. 2021 Apr 23;6(4):1621-1635. doi: 10.1021/acssensors.1c00166. Epub 2021 Apr 1.
Recently, , the keystone pathogen implicated in the development of gum disease (periodontitis), was detected in the brains of Alzheimer's disease patients, opening up a fascinating possibility that it is also involved in the pathobiology of this neurodegenerative illness. To verify this hypothesis, an unbiased, specific, and sensitive method to detect this pathogen in biological specimens is needed. To this end, our interdisciplinary studies demonstrate that can be easily identified by surface-enhanced Raman scattering (SERS). Moreover, based on SERS measurements, can be distinguished from another common periodontal pathogen, , and also from ubiquitous oral spp. The results were confirmed by principal component analysis (PCA). Furthermore, we have shown that different and strains can easily adsorb to silver-coated magnetic nanoparticles (FeO@AgNPs). Thus, it is possible to magnetically separate investigated bacteria from other components of a specimen using the microfluidic chip. To obtain additional enhancement of the Raman signal, the NPs adsorbed to bacterial cells were magnetically attracted to the Si/Ag SERS platform. Afterward, the SERS spectra could be recorded. Such a time-saving procedure can be very helpful in rapid medical diagnostics and thus in starting the appropriate pharmacological therapy to prevent the development of periodontitis and associated comorbidities, e.g., Alzheimer's disease.
最近，在阿尔茨海默病患者的大脑中检测到了与牙龈疾病（牙周炎）发展相关的关键病原体，这开启了一种引人入胜的可能性，即它也参与了这种神经退行性疾病的病理生物学过程。为了验证这一假设，需要一种无偏倚、特异性和灵敏的方法来在生物样本中检测这种病原体。为此，我们的跨学科研究表明，可以通过表面增强拉曼散射（SERS）轻松识别[病原体名称未给出]。此外，基于SERS测量，[病原体名称未给出]可以与另一种常见的牙周病原体[另一种病原体名称未给出]区分开来，也可以与普遍存在的口腔[菌种名称未给出]区分开来。结果通过主成分分析（PCA）得到了证实。此外，我们已经表明，不同的[病原体名称未给出]和[病原体名称未给出]菌株可以很容易地吸附到涂银磁性纳米颗粒（FeO@AgNPs）上。因此，有可能使用微流控芯片从样本的其他成分中磁性分离出所研究的细菌。为了进一步增强拉曼信号，吸附在细菌细胞上的纳米颗粒被磁性吸引到Si/Ag SERS平台上。之后，可以记录SERS光谱。这样一个节省时间的程序在快速医学诊断中可能非常有帮助，从而有助于启动适当的药物治疗，以预防牙周炎和相关合并症（如阿尔茨海默病）的发展。
