Department of Microbiology, University of Lagos, Akoka, Lagos state, Nigeria.
Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, Nigeria.
J Infect Dev Ctries. 2020 Feb 29;14(2):162-168. doi: 10.3855/jidc.11746.
Helicobacter pylori is a principal cause of gastric cancer. The aim of this study was to determine the prevalence and contribution of duodenal ulcer promoting gene A (dupA), the plasticity region genes and sigma factors in relation to their pathological expression of H. pylori infections in the Nigerian population.
Polymerase Chain Reaction was used to analyze a total of forty-nine H. pylori strains isolated from patients attending various endoscopic units in tertiary hospitals in Nigeria for complete dupA (G27 variant), jhp0917, jhp0918, other plasticity region genes jhp 914/917, jhp0914, jhp0940 and sigma factors.
PCR results indicated that the prevalence of complete dupA (G27 variants), jhp0917, jhp0918 and other plasticity region genes jhp0914, jhp0914/0917 and jhp0940 in the H. pylori strains were 4%, 53%, 88%, 73%, 12% and 0% respectively. The prevalence values of the sigma factors were 96%, 92%, 80% for rpoN, fliA and rpoD respectively. However, the endoscopic findings showed that erosion, normal mucosal, ulcer, hyperaemic stomach, mucosal atrophy and oedematous stomach in the patients where the H. pylori strains were isolated were 40.8%, 32.7%, 10.2%, 8.2%, 2.0% and 6.1% respectively. There was significant association between jhp0917, jhp914/917 and G27 variant and the endoscopic findings, while other plasticity genes showed no association with the endoscopic findings.
These results suggest that the presence of jhp0917, jhp0914/917 and G27 variant could be used as marker to predict the pathological effect of severity in Nigeria patients with H. pylori infection.
幽门螺杆菌是胃癌的主要病因。本研究旨在确定十二指肠溃疡促进基因 A（dupA）、可塑性区域基因和 sigma 因子在尼日利亚人群中与幽门螺杆菌感染的病理表现之间的流行率和相关性。
聚合酶链反应（PCR）用于分析从尼日利亚各三级医院内镜科就诊的 49 株幽门螺杆菌株，分析其完整的 dupA（G27 变异）、jhp0917、jhp0918、其他可塑性区域基因 jhp 914/917、jhp0914、jhp0940 和 sigma 因子。
PCR 结果表明，完整的 dupA（G27 变异）、jhp0917、jhp0918 和其他可塑性区域基因 jhp0914、jhp0914/0917 和 jhp0940 在幽门螺杆菌株中的流行率分别为 4%、53%、88%、73%、12%和 0%。sigma 因子的流行率分别为 rpoN、fliA 和 rpoD 的 96%、92%和 80%。然而，内镜检查结果显示，在分离出幽门螺杆菌株的患者中，侵蚀、正常黏膜、溃疡、充血性胃、黏膜萎缩和水肿性胃的发生率分别为 40.8%、32.7%、10.2%、8.2%、2.0%和 6.1%。jhp0917、jhp914/917 和 G27 变异与内镜检查结果有显著相关性，而其他可塑性基因与内镜检查结果无相关性。
这些结果表明，jhp0917、jhp0914/917 和 G27 变异的存在可作为预测尼日利亚幽门螺杆菌感染患者严重程度病理效应的标志物。
