The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, USA.
Escola Superior de Agricultura Luiz de Queiroz/Esalq/USP, Piracicaba, SP, Brazil.
Arch Virol. 2020 May;165(5):1225-1229. doi: 10.1007/s00705-020-04569-8. Epub 2020 Mar 7.
Using a high-throughput sequencing approach, we identified four genomoviruses (family Genomoviridae) associated with a sweet orange (Citrus sinensis) plant collected in Tunisia. The ssDNA genomes of these genomoviruses, which were amplified, cloned and Sanger sequenced, range in size from 2156 to 2191 nt. Three of these viruses share > 99% full-genome pairwise sequence identity and are referred to as citrus Tunisia genomovirus 1 (CTNGmV-1). The CTNGmV-1 isolates share < 62% genome-wide pairwise nucleotide sequence identity with other genomoviruses and belong to the genus Gemykolovirus. The genome of the fourth virus, which was called CTNGmV-2, shares < 68% nucleotide sequence identity with other genomoviruses and belongs to the genus Gemycircularvirus. Based on the species demarcation criteria for members of the family Genomoviridae, CTNGmV-1 and -2 would each represent a new species. Although found associated with Citrus sp. plants, it is likely that these viruses infect fungi or other organisms associated with the plants.
我们采用高通量测序方法，从采集自突尼斯的甜橙（Citrus sinensis）植株中鉴定出了 4 种与植物基因组相关的病毒（基因组病毒科）。这些基因组病毒的 ssDNA 基因组经扩增、克隆和 Sanger 测序后，大小在 2156 到 2191 个核苷酸之间。其中 3 种病毒的全基因组序列相似度超过 99%，被称为柑橘突尼斯基因组病毒 1（CTNGmV-1）。CTNGmV-1 分离株与其他基因组病毒的基因组-wide 核苷酸序列相似度<62%，属于 Gemykolovirus 属。第 4 种病毒被命名为 CTNGmV-2，其与其他基因组病毒的核苷酸序列相似度<68%，属于 Gemycircularvirus 属。根据基因组病毒科成员的种分类标准，CTNGmV-1 和 -2 各自代表一个新种。尽管 CTNGmV-1 和 -2 与柑橘属植物相关，但它们很可能感染与这些植物相关的真菌或其他生物体。
