鉴定植物病原菌葡萄孢中的一种新型基因组病毒。

Characterization of a novel genomovirus in the phytopathogenic fungus Botrytis cinerea.

机构信息

The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Institute of Vegetables and the Key Lab of Cucurbitaceous Vegetables Breeding in Ningbo City, Ningbo Academy of Agricultural Sciences, Ningbo, 315040, PR China.

出版信息

Virology. 2021 Jan 15;553:111-116. doi: 10.1016/j.virol.2020.11.007. Epub 2020 Nov 24.

DOI:10.1016/j.virol.2020.11.007

PMID:33264653

Abstract

This study characterized a single-stranded circular DNA virus in Botrytis cinerea-namely, Botrytis cinerea genomovirus 1 (BcGV1). The genome of BcGV1 was 1710 nucleotides (nts) long, possessing two ORFs, encoding a putative replication initiation protein (Rep) and a hypothetical protein. The Rep contained seven conserved motifs. The two ORFs were separated by two intergenic regions; the large intergenic region (LIR) contained 259 nts while the small intergenic region (SIR) contained 95 nts. A nonanucleotide, TAACAGTAC, in the LIR was predicted to be associated with the initiation of viral replication. Based on the phylogenetic tree constructed by Reps, BcGV1 belongs to the family Genomoviridae, forming an independent branch, indicating that BcGV1 may belong to a new genus. BcGV1 could be detected in 6.7% of tested B. cinerea strains, suggesting that BcGV1 may be widely distributed in the Chinese B. cinerea population.

摘要

本研究对 Botrytis cinerea 中的一种单链环状 DNA 病毒进行了特征描述，即 Botrytis cinerea 基因组病毒 1（BcGV1）。BcGV1 的基因组长 1710 个核苷酸（nts），包含两个开放阅读框，分别编码一个假定的复制起始蛋白（Rep）和一个假定蛋白。Rep 包含七个保守基序。两个 ORF 由两个基因间区隔开；大基因间区（LIR）包含 259 个核苷酸，小基因间区（SIR）包含 95 个核苷酸。LIR 中的非核苷酸 TAACAGTAC 被预测与病毒复制的起始有关。基于 Reps 构建的系统发育树，BcGV1 属于基因组病毒科，形成一个独立的分支，表明 BcGV1 可能属于一个新属。在中国 Botrytis cinerea 群体中，BcGV1 可在 6.7%的测试菌株中检测到，表明 BcGV1 可能广泛分布。