Nakasu Erich Y T, Melo Fernando L, Michereff-Filho Miguel, Nagata Tatsuya, Ribeiro Bergmann M, Ribeiro Simone G, Lacorte Cristiano, Inoue-Nagata Alice K
Embrapa Vegetables, Rodovia BR-060, Km09, Brasília, 70275-970, Brazil.
Department of Cell Biology, University of Brasilia, Brasília, 70910-900, Brazil.
Arch Virol. 2017 Sep;162(9):2835-2838. doi: 10.1007/s00705-017-3425-y. Epub 2017 May 31.
The complete genome sequences of two novel small circular DNA viruses isolated from sweet-potato whiteflies collected in central-West (AdDF) and Southeast (AdO) regions of Brazil were determined by Next Generation Sequencing (NGS), and confirmed by cloning and Sanger sequencing. The genomes are 2,199 and 2,211 nt-long, respectively, encoding a putative coat protein (CP) and a replication-associated protein (Rep) and showing a genomic organization typical of viruses from the family Genomoviridae. Phylogenetic analysis with deduced amino acid sequences of Rep indicates that the virus from AdO is closely related to other members of the genus Gemycircularvirus, while the virus from AdDF is distantly related to other genomovirus. It was thus classified in a putative new genus, for which the name "Gemybolavirus" is proposed. These new genomoviruses are tentatively named "Bemisia associated gemybolavirus AdDF", and "Bemisia associated gemycircularvirus AdO".
通过下一代测序（NGS）确定了从巴西中西部（AdDF）和东南部（AdO）地区采集的甘薯粉虱中分离出的两种新型小环状DNA病毒的完整基因组序列，并通过克隆和桑格测序进行了确认。这些基因组分别长2199和2211个核苷酸，编码一种假定的衣壳蛋白（CP）和一种复制相关蛋白（Rep），并显示出基因组病毒科病毒典型的基因组结构。对Rep推导氨基酸序列的系统发育分析表明，来自AdO的病毒与Gemycircularvirus属的其他成员密切相关，而来自AdDF的病毒与其他基因组病毒关系较远。因此，它被归类到一个假定的新属，为此提出了“Gemybolavirus”这个名称。这些新的基因组病毒暂命名为“粉虱相关Gemybolavirus AdDF”和“粉虱相关Gemycircularvirus AdO”。
