没药提取物对雄性糖尿病大鼠精子发生和睾酮水平的影响。

The effects of Commiphora mukul extract on spermatogenesis and testosterone levels in male diabetic rats.

作者信息

Rezaei Ali Akbar, Salehi Iraj, Karimi Seyed Asaad, Rahnama Mehdi

机构信息

Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Department of Physiology, Faculty of Science, Science and Research Branch, Islamic Azad University, Zanjan, Iran.

出版信息

Clin Exp Reprod Med. 2020 Mar;47(1):34-41. doi: 10.5653/cerm.2019.03251. Epub 2020 Mar 1.

DOI:10.5653/cerm.2019.03251

PMID:32146773

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7127907/

Abstract

OBJECTIVE

The strong antioxidant activity of Commiphora mukul prompted us to conduct the present study to explore whether treatment with C. mukul extract (CME) would have any protective influence on sperm parameters, testosterone levels, and plasma glucose levels in streptozotocin (STZ)-induced diabetic rats.

METHODS

Male Wistar rats were randomly divided into four groups: control, control animals treated with CME, diabetic animals, and diabetic animals treated with CME. CME extract (300 mg/kg) was administered for 60 days by daily gavage. Diabetes was induced by an intraperitoneal injection of 50 mg/kg STZ. The epididymal sperm count, weight, motility, morphology, viability, and serum testosterone and glucose levels were determined.

RESULTS

In the diabetic animals, CME decreased blood glucose levels (p<0.05), increased the total sperm count (p<0.05), and decreased the proportion of sperm with abnormal morphology (p<0.05). Diabetes reduced sperm motility (p<0.001), and CME supplementation partially reversed this effect of diabetes (p=0.003). Furthermore, in diabetic animals, CME decreased the proportion of immotile sperm (p<0.001). In rats, diabetes caused a significant decrease (p<0.05) in serum testosterone levels (F[3, 28]=3.283, p=0.035), but treatment of diabetic animals with CME increased serum testosterone levels.

CONCLUSION

The present study demonstrated that C. mukul possesses proandrogenic activity and exerts a beneficial effect on sperm parameters in diabetic rats.

摘要

目的

没药具有强大的抗氧化活性，促使我们开展本研究，以探讨没药提取物（CME）治疗对链脲佐菌素（STZ）诱导的糖尿病大鼠的精子参数、睾酮水平和血糖水平是否有任何保护作用。

方法

将雄性Wistar大鼠随机分为四组：对照组、接受CME治疗的对照动物组、糖尿病动物组和接受CME治疗的糖尿病动物组。通过每日灌胃给予CME提取物（300毫克/千克），持续60天。通过腹腔注射50毫克/千克STZ诱导糖尿病。测定附睾精子计数、重量、活力、形态、存活率以及血清睾酮和血糖水平。

结果

在糖尿病动物中，CME降低了血糖水平（p<0.05），增加了精子总数（p<0.05），并降低了形态异常精子的比例（p<0.05）。糖尿病降低了精子活力（p<0.001），补充CME部分逆转了糖尿病的这种作用（p=0.003）。此外，在糖尿病动物中，CME降低了不动精子的比例（p<0.001）。在大鼠中，糖尿病导致血清睾酮水平显著降低（p<0.05）（F[3, 28]=3.283，p=0.035），但用CME治疗糖尿病动物可提高血清睾酮水平。