Ghanbari Elham, Nejati Vahid, Najafi Gholamreza, Khazaei Mozafar, Babaei Mohammad
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.
Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Int J Fertil Steril. 2015 Apr-Jun;9(1):113-20. doi: 10.22074/ijfs.2015.4215. Epub 2015 Apr 21.
Diabetes mellitus has a variety of structural and functional effects on the male reproductive system. Diabetes results in reduced sperm parameters and libido. The present study aims to investigate the effects of royal jelly (RJ) on reproductive parameters of testosterone and malondialdehyde (MDA) production in diabetic rats.
This experimental study was conducted on adult male Wistar rats. The animals were divided into four groups (n=8 per group): control, RJ, diabetic and diabetic treated with RJ. Diabetes was induced by intraperitoneal injection of 60 mg/kg body weight (BW) of streptozotocin (STZ). RJ, at a dose of 100 mg/kg BW was given by gavage. The duration of treatment was six weeks. After the treatment period the rats were sacrificed. The testes were weighed and changes in sperm count, motility, viability, deformity, DNA integrity and chromatin quality were analyzed. Serum testosterone and MDA concentrations of testicular tissue were determined. Data were analyzed by oneway ANOVA with p<0.05 as the significant level.
STZ-induced diabetes decreased numerous reproductive parameters in rats. Testicular weight, sperm count, motility, viability and serum testosterone levels increased in the diabetic group treated with RJ. There was a significant decrease observed in sperm deformity, DNA integrity, chromatin quality, and tissue MDA levels in diabetic rats treated with RJ compared to the diabetic group (p<0.05).
RJ improved reproductive parameters such as testicular weight, sperm count, viability, motility, deformity, DNA integrity, chromatin quality, serum testosterone and testicular tissue MDA levels in diabetic rats.
糖尿病对男性生殖系统具有多种结构和功能影响。糖尿病会导致精子参数和性欲降低。本研究旨在探讨蜂王浆（RJ）对糖尿病大鼠睾酮生殖参数及丙二醛（MDA）生成的影响。
本实验研究以成年雄性Wistar大鼠为对象。动物被分为四组（每组n = 8）：对照组、RJ组、糖尿病组和糖尿病RJ治疗组。通过腹腔注射60 mg/kg体重的链脲佐菌素（STZ）诱导糖尿病。以100 mg/kg体重的剂量通过灌胃给予RJ。治疗持续时间为六周。治疗期结束后处死大鼠。称量睾丸重量，并分析精子数量、活力、存活率、畸形率、DNA完整性和染色质质量的变化。测定睾丸组织的血清睾酮和MDA浓度。数据采用单因素方差分析进行分析，以p<0.05为显著水平。
STZ诱导的糖尿病降低了大鼠的多项生殖参数。RJ治疗的糖尿病组睾丸重量、精子数量、活力、存活率和血清睾酮水平增加。与糖尿病组相比，RJ治疗的糖尿病大鼠精子畸形率、DNA完整性、染色质质量和组织MDA水平显著降低（p<0.05）。
RJ改善了糖尿病大鼠的生殖参数，如睾丸重量、精子数量、存活率、活力、畸形率、DNA完整性、染色质质量、血清睾酮和睾丸组织MDA水平。
