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粘质沙雷氏菌的血清分型:勒米诺H固定试验的评估及三种新的鞭毛H抗原的描述

Serotyping of Serratia marcescens: evaluation of Le Minor's H-immobilization test and description of three new flagellar H antigens.

作者信息

Traub W H, Kleber I

出版信息

J Clin Microbiol. 1977 Feb;5(2):115-21. doi: 10.1128/jcm.5.2.115-121.1977.

Abstract

The H-immobilization test of Le Minor for determining flagellar (H) antigens was evaluated and compared with tube and slide H-agglutination tests. The test proved specific and easy to perform, and titration end points were clearly discernible. The degree of serological cross-reactivity between H antigen reference strains of Serratia marcescens was low. Consequently, this test was adopted for routine serological analysis of H antigens, using unabsorbed rabbit immune anti-H sera. As a result of using this procedure, three new provisional H antignes, designated H14, H15, and H16, are proposed.

摘要

对用于确定鞭毛(H)抗原的勒米诺尔H固定试验进行了评估,并与试管和玻片H凝集试验进行了比较。该试验证明具有特异性且易于操作,滴定终点清晰可辨。粘质沙雷氏菌H抗原参考菌株之间的血清学交叉反应程度较低。因此,该试验被用于使用未吸收的兔免疫抗H血清对H抗原进行常规血清学分析。采用该方法的结果是,提出了三种新的临时H抗原,分别命名为H14、H15和H16。

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