Farmer J J
Appl Microbiol. 1972 Feb;23(2):218-25. doi: 10.1128/am.23.2.218-225.1972.
A new method for comparing and differentiating strains of S. marcescens is described which has proved useful in determining the epidemiology of hospital infections. Strains were grown in Trypticase soy broth, and bacteriocin production was induced with mitomycin C for 5 hr. The bacteriocin lysates were then spotted onto nine standard indicator strains, which were chosen with the aid of computer analysis from the 118 indicators tested. After 24 hr at 37 C, zones of inhibition due to bacteriocins were recorded. One hundred twentynine strains were differentiated into 72 different bacteriocin production patterns, but 11 strains were nontypable. None of the 45 other strains of Enterobacteriaceae produced bacteriocins. Bacteriocin production was a stable epidemiological marker. Colonial mutants always had identical patterns, as did the same strain which has passed from patient to patient through cross-infection. The new technique does not require any specialized equipment and can be used in laboratories with limited budgets. The applications of the new method in cross-infection studies and as a supplement to serological typing are discussed.
本文描述了一种比较和区分粘质沙雷氏菌菌株的新方法，该方法已被证明在确定医院感染的流行病学方面很有用。将菌株接种于胰蛋白胨大豆肉汤中，用丝裂霉素C诱导细菌素产生5小时。然后将细菌素裂解物点种到9种标准指示菌株上，这些指示菌株是通过计算机分析从118种受试指示菌中挑选出来的。在37℃培养24小时后，记录由细菌素引起的抑菌圈。129株菌株被分为72种不同的细菌素产生模式，但有11株无法分型。其他45株肠杆菌科菌株均不产生细菌素。细菌素的产生是一种稳定的流行病学标记。菌落突变体总是具有相同的模式，通过交叉感染在患者之间传播的同一菌株也是如此。这项新技术不需要任何专门设备，可在预算有限的实验室中使用。本文还讨论了该新方法在交叉感染研究中的应用以及作为血清学分型补充方法的应用。
