Hisanaga K, Tsukui H, Takei N, Hatanaka H
Department of Neuroscience, Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Brain Res. 1988 Dec 20;475(2):349-55. doi: 10.1016/0006-8993(88)90624-5.
Fetal rat (E17-E19) septal neurons were cultured in a defined, serum-free medium for 6-8 days with or without nerve growth factor (NGF) and transplanted into the hippocampus or the surrounding ventricle of 28 adult rats denervated of its septal input by a fimbria-fornix transection. The cholinergic septal neurons, which were visualized by acetylcholinesterase (AChE) histochemistry, always survived in transplantation to the adult brains from nearly pure neuronal cultures. Although choline acetyltransferase (ChAT) activity of septal neurons in culture was greatly increased (5.59-fold) by the addition of NGF to the defined medium, this ChAT induction appeared to have little effect on the subsequent survival or growth of the septal neurons after transplantation. These results demonstrate that survival of cultured fetal septal cholinergic neurons following transplantation is not dependent upon the presence of NGF or serum- or glia-derived factors during the preliminary culture. Postnatal rat (P4) septal neurons cultured for 5 days in serum-containing medium with NGF were also successfully transplanted in one of 3 cases.
将胎鼠（胚胎第17 - 19天）的隔区神经元在特定的无血清培养基中培养6 - 8天，添加或不添加神经生长因子（NGF），然后移植到28只成年大鼠的海马体或其周围脑室，这些成年大鼠通过穹窿 - 海马伞横断术切断了隔区输入。通过乙酰胆碱酯酶（AChE）组织化学法可视化的胆碱能隔区神经元，在从近乎纯的神经元培养物移植到成年大脑中时总能存活下来。虽然在特定培养基中添加NGF可使培养的隔区神经元的胆碱乙酰转移酶（ChAT）活性大幅增加（5.59倍），但这种ChAT诱导似乎对移植后隔区神经元的后续存活或生长影响很小。这些结果表明，培养的胎儿隔区胆碱能神经元移植后的存活并不依赖于在初步培养期间是否存在NGF或血清或神经胶质细胞衍生的因子。在含血清培养基中添加NGF培养5天的新生大鼠（出生后第4天）隔区神经元，在3例中有1例也成功进行了移植。
