Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, P. R. China.
Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.
J Mater Chem B. 2020 Apr 1;8(13):2673-2688. doi: 10.1039/c9tb02864a.
An effective treatment for spinal cord injury (SCI) remains a severe clinical challenge due to the intrinsically limited regenerative capacity and complex anatomical structure of the spinal cord. The combination of biomaterials, which serve as scaffolds for axonal growth, cells and neurotrophic factors, is an excellent candidate for spinal cord regeneration. Herein, a new micropatterned conductive electrospun nanofiber mesh was constructed with poly{[aniline tetramer methacrylamide]-co-[dopamine methacrylamide]-co-[poly(ethylene glycol) methyl ether methacrylate]}/PCL (PCAT) using a rotation electrospinning technology. The aim was to study the synergistic effects of electrical stimulation (ES) and a micropatterned conductive electrospun nanofiber mesh incorporated with nerve growth factor (NGF) on the differentiation of rat nerve stem cells (NSCs). The hydrophilicity of the conductive nanofiber mesh could be tailored by changing the dopamine (DA) and aniline tetramer (AT) content from 19° to 79°. A favorable electroactivity and conductivity was achieved by the AT segment of PCAT. The as-fabricated micropatterned electrospun nanofiber mesh possessed a regularly aligned valley and ridge structure, and the diameter of the nanofiber was 312 ± 58 nm, while the width of the valley and ridge was measured to be 210 ± 17 μm and 200 ± 16 μm, respectively. The growth and neurite outgrowth of differentiated NSCs were observed along the valley of the micropatterned nanofiber mesh. In addition, the NGF loaded micropatterned conductive electrospun nanofiber mesh combined with ES exhibited the highest cell viability, and effectively facilitated the differentiation of NSCs into neurons and suppressed the formation of astrocytes, thus exhibiting a great application potential for nerve tissue engineering.
一种有效的脊髓损伤（SCI）治疗方法仍然是一个严重的临床挑战，因为脊髓本身的再生能力有限，解剖结构复杂。生物材料与细胞和神经营养因子结合，作为轴突生长的支架，是脊髓再生的一个极好的候选物。在此，通过旋转静电纺丝技术，使用聚{[苯胺四聚体甲基丙烯酰胺]-共-[多巴胺甲基丙烯酰胺]-共-[聚（乙二醇）甲醚甲基丙烯酸酯]}/PCL（PCAT）构建了一种新的微图案化导电静电纺纳米纤维网。目的是研究电刺激（ES）和结合神经生长因子（NGF）的微图案化导电静电纺纳米纤维网对大鼠神经干细胞（NSCs）分化的协同作用。通过改变多巴胺（DA）和苯胺四聚体（AT）的含量，可将导电纳米纤维网的亲水性从 19°调整到 79°。PCAT 的 AT 段可实现良好的电活性和导电性。所制备的微图案化静电纺纳米纤维网具有规则排列的山谷和脊结构，纳米纤维的直径为 312±58nm，而山谷和脊的宽度分别测量为 210±17μm和 200±16μm。分化后的 NSCs 的生长和神经突延伸沿着微图案化纳米纤维网的山谷进行。此外，负载 NGF 的微图案化导电静电纺纳米纤维网与 ES 结合表现出最高的细胞活力，并有效地促进了 NSCs 向神经元的分化，抑制了星形胶质细胞的形成，因此在神经组织工程中具有很大的应用潜力。
